Department of Food and Nutrition, Tsu City College, Tsu, Japan.
Faculty of Health Science, Suzuka University of Medical Science, Suzuka, Japan.
J Appl Physiol (1985). 2022 May 1;132(5):1204-1212. doi: 10.1152/japplphysiol.00789.2021. Epub 2022 Mar 31.
We examined the effects and interactions of a low-carbohydrate, high-fat (LCHF) diet and voluntary running exercise on bone in older mice. Male 19-mo-old mice were divided into four groups by diet (control vs. LCHF) and exercise (sedentary vs. voluntary running). The control diet was 55% carbohydrate, 23% protein, and 22% fat, and the LCHF diet was 10% carbohydrate, 33% protein, and 57% fat as percentages of calories. The experiment ended when the mice reached 24 mo old. Statistical analysis was conducted using two-way analysis of variance with diet and exercise. The LCHF diet decreased bone mineral content (BMC), bone mineral density, bone volume fraction, and trabecular number. There was no significant interaction between diet and exercise on many bone parameters. However, there were significant diet and exercise interactions on lumbar BMC and tibial trabecular total tissue volume and average cortical thickness. The LCHF diet attenuated the benefit of running exercise on lumbar BMC and caused running to have a negative effect on tibial trabecular total tissue volume. Our study suggests that the LCHF diet impairs bone mass and some trabecular microstructure and reduces the benefit of exercise on lumbar BMC in old mice. An LCHF diet is used in treatment and prevention of diseases or improving exercise performance. However, some studies have shown that an LCHF diet diminishes bone in young rodents. Our study demonstrates that an LCHF diet impairs bone mass and some trabecular microstructure in old mice, which are similar to the previous studies using young rodents. Moreover, our study shows that an LCHF diet reduces the benefit of exercise on lumbar BMC in old mice.
我们研究了低碳水化合物高脂肪（LCHF）饮食和自愿跑步运动对老年小鼠骨骼的影响及其相互作用。雄性 19 月龄小鼠按饮食（对照 vs. LCHF）和运动（久坐不动 vs. 自愿跑步）分为四组。对照饮食中碳水化合物占 55%，蛋白质占 23%，脂肪占 22%，而 LCHF 饮食中碳水化合物占 10%，蛋白质占 33%，脂肪占 57%，均为卡路里的百分比。当小鼠达到 24 月龄时，实验结束。使用双因素方差分析对数据进行统计分析，包括饮食和运动。LCHF 饮食降低了骨矿物质含量（BMC）、骨密度、骨体积分数和小梁数量。饮食和运动对许多骨骼参数没有显著的相互作用。然而，在腰椎 BMC 和胫骨小梁总组织体积和平均皮质厚度方面，饮食和运动存在显著的相互作用。LCHF 饮食减弱了运动对腰椎 BMC 的益处，并导致运动对胫骨小梁总组织体积产生负面影响。我们的研究表明，LCHF 饮食会损害骨量和一些小梁微观结构，并降低运动对老年小鼠腰椎 BMC 的益处。LCHF 饮食用于治疗和预防疾病或提高运动表现。然而，一些研究表明，LCHF 饮食会减少年轻啮齿动物的骨量。我们的研究表明，LCHF 饮食会损害老年小鼠的骨量和一些小梁微观结构，这与之前使用年轻啮齿动物的研究结果相似。此外，我们的研究表明，LCHF 饮食会降低运动对老年小鼠腰椎 BMC 的益处。
