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基于米糠发酵生产结冷胶及其胶的各种定性性质评估。

The fermentation-based production of gellan from rice bran and the evaluation of various qualitative properties of gum.

作者信息

Kamer Deniz Damla Altan, Gumus Tuncay, Palabiyik Ibrahim, Demirci Ahmet Sukru, Oksuz Omer

机构信息

Department of Food Engineering, Tekirdag Namik Kemal University, 59030 Tekirdag, Turkey.

Department of Food Engineering, Tekirdag Namik Kemal University, 59030 Tekirdag, Turkey.

出版信息

Int J Biol Macromol. 2022 May 15;207:841-849. doi: 10.1016/j.ijbiomac.2022.03.168. Epub 2022 Mar 28.

DOI:10.1016/j.ijbiomac.2022.03.168
PMID:35358576
Abstract

The potential for the use of rice bran, an agricultural waste, as a substrate in the manufacture of gellan gum was examined. Using a standard strain of Sphingomonas paucimobilis (ATCC 31461) and rice bran substrate, gellan gum was produced under optimized conditions. The optimal yield of gellan gum using rice bran substrate was found to be 11.96 g L with 5% glucose, 10% inoculum, and a mixing speed of 300 rpm. Native gum was found to have a consistency index of 2.00 Pa.s. The viscosity of the gum was found to be extremely stable when exposed to thermal stress. Concerning the rheological characteristics, the Herschel-Bulkley model offered a more realistic representation of the flow characteristics of gum solutions. The synthesized gums were mostly composed of glucose, rhamnose, and glucuronic acid. The acetic acid content of gellan gums was 2.95%, while the molecular weight was 2.88 × 10 Da. Characterization of native gellan gums by UV-Vis spectroscopy, SEM, TEM and FTIR spectroscopy is also presented.

摘要

研究了将农业废弃物米糠用作结冷胶生产底物的可能性。使用少动鞘氨醇单胞菌标准菌株(ATCC 31461)和米糠底物,在优化条件下生产结冷胶。发现使用米糠底物时,结冷胶的最佳产量为11.96 g/L,葡萄糖含量为5%,接种量为10%,混合速度为300 rpm。发现天然胶的稠度指数为2.00 Pa·s。当暴露于热应力时,该胶的粘度极其稳定。关于流变特性,赫谢尔-布尔克利模型能更真实地反映胶溶液的流动特性。合成的胶主要由葡萄糖、鼠李糖和葡萄糖醛酸组成。结冷胶的乙酸含量为2.95%,分子量为2.88×10 Da。还介绍了通过紫外-可见光谱、扫描电子显微镜、透射电子显微镜和傅里叶变换红外光谱对天然结冷胶的表征。

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