Molecular Endocrinology, Functional Genomics & Systems Biology Laboratory, Animal Biochemistry Division, ICAR - National Dairy Research Institute, Karnal, India.
Molecular Endocrinology, Functional Genomics & Systems Biology Laboratory, Animal Biochemistry Division, ICAR - National Dairy Research Institute, Karnal, India.
Gene. 2022 Jun 15;827:146456. doi: 10.1016/j.gene.2022.146456. Epub 2022 Mar 28.
Postpartum anestrus interval (PPAI) is the interval between parturition and the first postpartum estrus exhibition in animals. Appearance of both normal and PPA buffaloes under the same farm conditions indicates the role of possible genetic predisposition to PPA. To identify the genetic and non-genetic factors associated with PPA in buffaloes, we collected data on PPAI and other 38 non-genetic variables from 575 Murrah buffaloes in the field conditions and identified the PPA associated non-genetic factors in our previous study. To explore the genetic factors associated with the unexplained variation in PPAI residuals, the present study identified 41 single nucleotide polymorphisms (SNPs) in 13 candidate genes using Sanger sequencing. Exploration of their association with the PPAI residuals of 50 extreme PPA and 50 normal buffaloes identified the significant (P < 0.01) association of the SNP (g.37219977A>G) in the 3'-UTR region of the Meprin A 1 subunit beta (Mep1b) gene with PPAI, which was further validated (P = 0.058) in a large population sample (n = 417). Bioinformatics analysis of the 3'-UTR region has identified three miRNA, bta-miR-2420, bta-miR-2325b and bta-miR-453 that could regulate Igf-1 in the plasma of animals with different genotypes (GG, AG and AA). The higher Igf-1 levels in the GG genotypes than that of AA and AG genotypes of this SNP (g.37219977A>G) further suggest the association of Mep1b gene with PPA condition in Murrah buffaloes. As a result of this study, we propose that buffaloes with protective alleles at this SNP be selected to improve the herd's reproductive efficiency.
产后乏情期（PPAI）是指动物分娩后至首次产后发情的间隔时间。在相同的农场条件下，正常和 PPA 水牛的出现表明 PPA 可能存在遗传倾向。为了确定与水牛 PPA 相关的遗传和非遗传因素，我们在田间条件下收集了 575 头摩拉水牛的 PPAI 和其他 38 个非遗传变量的数据，并在之前的研究中确定了与 PPA 相关的非遗传因素。为了探索与 PPAI 残差无关的遗传因素，本研究使用 Sanger 测序在 13 个候选基因中鉴定了 41 个单核苷酸多态性（SNP）。探索它们与 50 头极端 PPA 和 50 头正常水牛的 PPAI 残差的关联，确定了 Meprin A 1 亚基β（Mep1b）基因 3'-UTR 区域的 SNP（g.37219977A>G）与 PPAI 显著相关（P < 0.01），这在一个大的群体样本（n = 417）中得到了进一步验证（P = 0.058）。对 3'-UTR 区域的生物信息学分析确定了三个 miRNA，bta-miR-2420、bta-miR-2325b 和 bta-miR-453，它们可以调节不同基因型（GG、AG 和 AA）动物血浆中的 Igf-1。该 SNP（g.37219977A>G）的 GG 基因型中的 Igf-1 水平高于 AA 和 AG 基因型，进一步表明 Mep1b 基因与摩拉水牛的 PPA 状况有关。因此，我们建议选择具有这种 SNP 保护等位基因的水牛，以提高牛群的繁殖效率。
