Lokhorst H M, Boom S E, Bast B J, Ballieux R E
Br J Haematol. 1986 Oct;64(2):271-5. doi: 10.1111/j.1365-2141.1986.tb04119.x.
A new technique is described in which plasma cells actively synthesizing DNA can be identified in a heterogeneous cell population such as bone marrow. This method uses bromodeoxyuridine (BrdU) and a fluorescent monoclonal antibody against BrdU in combination with cytoplasmic staining for immunoglobulin (Ig). In 26 patients with monoclonal gammopathy (MG) the plasma cell labelling index (LI) as determined by this immunofluorescent method was compared with the tritiated thymidine (3H-TdR) LI. No difference in sensitivity was found between the two methods. As the determination of the plasma cell LI with the BrdU/anti BrdU method is easy to perform and results can be obtained within 4 h, this immunofluorescent method seems to be an attractive alternative to the laborious time-consuming classic 3H-TdR LI.
本文描述了一种新技术，利用该技术可在诸如骨髓等异质性细胞群体中识别出正在积极合成DNA的浆细胞。该方法使用溴脱氧尿苷（BrdU）和抗BrdU荧光单克隆抗体，并结合免疫球蛋白（Ig）的细胞质染色。对26例单克隆丙种球蛋白病（MG）患者，采用这种免疫荧光法测定的浆细胞标记指数（LI）与氚标记胸腺嘧啶核苷（3H-TdR）LI进行了比较。两种方法在敏感性上未发现差异。由于采用BrdU/抗BrdU方法测定浆细胞LI易于操作，且4小时内即可获得结果，因此这种免疫荧光法似乎是替代耗时费力的经典3H-TdR LI的一种有吸引力的方法。
