Laboratory of Protozoological Biodiversity and Evolution in Wetland, College of Life Sciences, Shaanxi Normal University, Xi'an 710119, China.
Department of Life Sciences, Natural History Museum, London SW7 5BD, United Kingdom.
Eur J Protistol. 2022 Jun;84:125879. doi: 10.1016/j.ejop.2022.125879. Epub 2022 Feb 24.
The morphogenesis of two hypotrich ciliates, Notohymena antarctica Foissner, 1996 and Engelmanniella mobilis (Engelmann, 1862) Foissner, 1982, were investigated using protargol staining. The main events of the oxytrichid Notohymena antarctica during binary fission are as follows: (1) the oral primordium of the opisthe is formed apokinetally left of transverse cirrus II/1; (2) six streaks of frontal-ventral-transverse cirral anlagen are segmented in the ordinary pattern 1: 3: 3: 3: 4: 4 from left to right, which form three frontal, four frontoventral, one buccal, three ventral, two pretransverse ventral and five transverse cirri; (3) marginal anlagen are formed intrakinetally and the right marginal anlagen develop much earlier than those on the left. Phylogenetic analyses based on SSU rDNA sequence data indicate that Notohymena is monophyletic. A remarkable morphogenetic feature in E. mobilis is the mode of formation of the right marginal rows. In this paper, we clarified that the right marginal anlagen of E. mobilis are formed and develop to the right of the parental outer right marginal row, and that the grandparental (inner) right marginal row and great-grand parental (the outermost) left marginal row are resorbed. It is the first time to report a population of E. mobilis with comprehensive information, i.e. the morphology, ontogeny and molecular sequence.
使用蛋白银染色法研究了两种旋口目纤毛虫（南极拟棘盘虫 Notohymena antarctica Foissner，1996 和移动兜甲藻 Engelmanniella mobilis（Engelmann，1862）Foissner，1982）的形态发生。在二分裂过程中，棘口目南极拟棘盘虫的主要事件如下：（1）口前纤毛原基在横向纤毛 II/1 的左侧向侧方形成；（2）按照通常的模式 1:3:3:3:4:4 从左到右顺序分割 6 条额腹横纤毛原基，形成 3 条额纤毛、4 条额腹纤毛、1 条口纤毛、3 条腹纤毛、2 条前横腹纤毛和 5 条横纤毛；（3）基体在向侧方形成，右侧基体的发育比左侧早得多。基于 18S rDNA 序列数据的系统发育分析表明，南极拟棘盘虫是单系的。E. mobilis 的一个显著形态发生特征是右缘列的形成方式。在本文中，我们澄清了 E. mobilis 的右缘基体是在亲代外右缘列的右侧形成并发育的，而祖代（内）右缘列和高祖代（最外侧）左缘列被吸收。这是首次报道具有全面信息（形态、个体发生和分子序列）的 E. mobilis 种群。
