Zhang Tengyue, Qi Hongli, Zhang Tengteng, Sheng Yalan, Warren Alan, Shao Chen
Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China; The Key Laboratory of Biomedical Information Engineering, Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China.
Eur J Protistol. 2018 Oct;66:48-62. doi: 10.1016/j.ejop.2018.07.004. Epub 2018 Aug 2.
The morphology, morphogenesis and small subunit (SSU) rRNA gene-based phylogeny of Neourostylopsis flava paraflava nov. subsp. were investigated. Neourostylopsis flava paraflava nov. subsp. was separated from N. flava flava nov. stat. by habitat (brackish water vs. fresh water), pretransverse cirri (absent in all specimens vs. present in 15% of specimens), the numbers of frontal cirri (8-15 vs. 6-8) and left marginal cirral rows (6-9 vs. 4-5). The main morphogenetic features of N. flava paraflava nov. subsp. are as follows: (1) streaks I-VI (or I-VIII, deduced from morphological data) produce the bicorona; (2) the oral primordium and frontoventral-transverse cirral anlagen in the opisthe are formed de novo on the cell surface; (3) the numerous macronuclear nodules fuse into a branch-like mass; (4) two pretransverse ventral cirri are formed initially but disappear in the later stages; and (5) some of the frontoventral-transverse cirral anlagen develop in a primary mode. Based on SSU rDNA sequence data, phylogenetic analyses show a close relationship between N. flava paraflava nov. subsp., N. flava flava nov. stat. and other Neourostylopsis species. An improved diagnosis for Neourostylopsis is provided: Urostylidae with five or more frontal cirri which form an indistinct or distinct bicorona; pretransverse cirri present or absent; transverse cirri present; buccal cirri present; two frontoterminal cirri; midventral complex composed of midventral pairs only; more than one row of marginal cirri on each side which derive from individual anlagen within each parental row; caudal cirri lacking; three dorsal kineties; endoral and paroral rather long, endoral straight, paroral distinctly curved anteriorly.
对新种黄新拟尾柱虫副黄亚种（Neourostylopsis flava paraflava nov. subsp.）的形态、形态发生及基于小亚基（SSU）rRNA基因的系统发育进行了研究。黄新拟尾柱虫副黄亚种与黄新拟尾柱虫新组合（N. flava flava nov. stat.）在生境（咸淡水与淡水）、前横纤毛（所有标本均无与15%的标本有）、额纤毛数量（8 - 15根与6 - 8根）以及左缘纤毛列数（6 - 9列与4 - 5列）方面存在差异。黄新拟尾柱虫副黄亚种的主要形态发生特征如下：（1）条纹I - VI（或根据形态数据推断为I - VIII）产生双冠状结构；（2）后仔虫中的口原基和额腹 - 横纤毛原基在细胞表面重新形成；（3）众多大核结节融合成分支状团块；（4）最初形成两根前腹横纤毛，但在后期消失；（5）一些额腹 - 横纤毛原基以初级模式发育。基于SSU rDNA序列数据的系统发育分析表明，黄新拟尾柱虫副黄亚种、黄新拟尾柱虫新组合与其他拟尾柱虫物种之间关系密切。提供了对拟尾柱虫的改进诊断：尾柱科，具5根或更多额纤毛，形成不明显或明显的双冠状结构；前横纤毛有或无；横纤毛有；颊纤毛有；两根额终纤毛；腹中部复合体仅由腹中部对组成；每侧有多于一排的缘纤毛，源自每个亲代列内的单个原基；无尾纤毛；有三列背动基列；口内膜和口旁膜相当长，口内膜直，口旁膜向前明显弯曲。
