Clapham David H, Dormling Ingegerd, Ekberg Lnger, Eriksson Gösta, Qamaruddin Mohammed, Vince-Prue Daphne
D. H. Clapham (corresponding author, e-mail
Physiol Plant. 1998 Jan;102(1):71-78. doi: 10.1034/j.1399-3054.1998.1020110.x.
To test for the effects of far-red light on preventing budset in Picea abies, seedlings of six populations originating from latitudes between 67°N and 47°N were grown for 4-8 weeks in continuous incandescent (metal halogen) light at 300 µmol m s and 20°C and then transferred, at the same temperature, to a daily regime of 8 h incandescent light (300 µmol m s ) followed by 16 h cool white fluorescent light (40 µmol m s ). (Cool white lamps are deficient in far-red light, with a R/FR ratio of 7.5 compared with 2.0 for the incandescent lamps.) All the seedlings from 67° and 80% of those from 64° stopped extension growth and set terminal buds within 28 days of the change of regime. The seedlings from 61° and further south continued growing, as did control seedlings from 67° grown as above but with incandescent light at 20 µmol m s replacing cool white illumination. To distinguish between a clinal and ecotypic pattern of variation, the interval between 64° and 59° was investigated by growing populations originating from that area in the same regimes as before. After 28 days in the cool white day-extension regime, the percentage budset was 86 for the population from 64°, 0 for the population from 59° and 25-50 for the intermediate populations; i.e. the populations showed a clinal variation in requirement for far-red light according to latitude. Thus northern populations of Picea abies appear to behave as 'light-dominant' plants for the photoperiodic control of extension growth and budset, whereas the more southern populations behave as 'dark-dominant' plants.
为了测试远红光对防止欧洲云杉芽形成的影响，将来自北纬67°至47°之间六个种群的幼苗，在300 μmol m⁻² s⁻¹的连续白炽灯（金属卤化物灯）光照和20°C条件下培养4 - 8周，然后在相同温度下，转移到每天8小时白炽灯光照（300 μmol m⁻² s⁻¹）随后16小时冷白色荧光灯光照（40 μmol m⁻² s⁻¹）的光照周期中。（冷白色灯缺乏远红光，其红/远红比值为7.5，而白炽灯的该比值为2.0。）来自67°的所有幼苗以及来自64°的80%的幼苗在光照周期改变后的28天内停止伸长生长并形成顶芽。来自61°及更南部地区的幼苗继续生长，同样，来自67°的对照幼苗，在上述条件下生长，但用20 μmol m⁻² s⁻¹的白炽灯光照代替冷白色光照，也继续生长。为了区分渐变和生态型变异模式，通过在与之前相同的光照周期中培养来自该区域的种群，对64°和59°之间的区域进行了研究。在冷白色日延长光照周期下28天后，来自64°的种群的芽形成百分比为86%，来自59°的种群为0，中间种群为25% - 50%；即这些种群根据纬度在对远红光的需求上呈现渐变变异。因此，欧洲云杉的北部种群在伸长生长和芽形成的光周期控制方面似乎表现为“光主导”植物，而更南部的种群则表现为“暗主导”植物。
