原位杂交鉴定淡水海绵 Ephydatia fluviatilis 中的干细胞。
In Situ Hybridization to Identify Stem Cells in the Freshwater Sponge Ephydatia fluviatilis.
机构信息
Department of Biophysics, Graduate School of Science, Kyoto University, Kitashirakawa-Oiwake, Sakyo-ku, Kyoto, Japan.
出版信息
Methods Mol Biol. 2022;2450:335-346. doi: 10.1007/978-1-0716-2172-1_17.
Sponges (Porifera) are a large phylum that includes an enormous number of species. They are classified into four classes. Among these four classes, class Demospongiae is the largest and contains more than 90% of sponge species. In the last decade, methodologies for molecular studies and sequencing resources in sponge biology have dramatically advanced and made it possible to clearly define particular types of cells based on the genes they are expressing. Here we describe in detail the method of high-resolution WISH (whole mount in situ hybridization) and dual color fluorescent detection of in situ hybridization (dual color FISH) that we have established to detect particular types of cells, especially their stem cells known as archeocytes, in juveniles of freshwater demosponge, E. fluviatilis.
海绵动物（Porifera）是一个大型门，包括大量的物种。它们被分为四个纲。在这四个纲中，寻常海绵纲是最大的，包含超过 90%的海绵物种。在过去的十年中，海绵生物学的分子研究方法和测序资源取得了巨大的进展，使得基于它们表达的基因来明确特定类型的细胞成为可能。在这里，我们详细描述了我们建立的高分辨率 WISH（整体原位杂交）和原位杂交的双色荧光检测（dual color FISH）方法，用于检测特定类型的细胞，特别是它们的干细胞，称为始原细胞，在淡水寻常海绵，E. fluviatilis 的幼体中。
Zotero 插件