Department of Molecular & Cell Biology, University of California, Merced, CA, USA.
Quantitative and Systems Biology Graduate Program, University of California, Merced, CA, USA.
Methods Mol Biol. 2022;2450:479-491. doi: 10.1007/978-1-0716-2172-1_25.
Comet assay provides the opportunity to detect and characterize DNA strand breaks. Cellular lysing followed by embedding in agarose slide is used to visualize under an electrical current migration patterns corresponding to DNA fragments of different sizes. Here we describe the process of detecting and characterizing DNA damage by Comet assay on planarians, which is a model organism commonly used to understand the process of whole-body regeneration, stem cell regulation, and adult tissue maintenance.
彗星实验提供了检测和表征 DNA 链断裂的机会。通过细胞裂解，然后将其嵌入琼脂糖载玻片，在电流迁移模式下可视化对应于不同大小 DNA 片段的模式。在这里，我们描述了使用彗星实验检测和表征涡虫 DNA 损伤的过程，涡虫是一种常用的模式生物，用于了解全身再生、干细胞调节和成年组织维持的过程。
