Rideau N, Simon J, Leclercq B
Endocrinology. 1986 Dec;119(6):2635-41. doi: 10.1210/endo-119-6-2635.
Insulin release from the perfused pancreas was studied in genetically selected fat and lean chickens. The previously described glucose insensitivity of the chicken pancreas cannot be overcome by 5 or 10 mM D-glyceraldehyde, suggesting that the resistance is not related to glucose metabolism before the triose phosphate step. At 42 mM, glucose induced a biphasic insulin release which was specific, since 42 mM mannitol did not elicit insulin release. Arginine (10 mM) or acetylcholine (0.1-1 microM), which in themselves do not cause insulin release, generated a biphasic insulin release in the presence of a low nonstimulating glucose concentration (14 mM); the effect was synergistic. In contrast to the glucose tolerance test observed in vivo, the pancreas from the fat line chicken in response to glucose or glucose plus arginine released significantly less insulin during the first phase. The significance of this defect awaits further elucidation. On the other hand, acetylcholine, a more potent secretagogue, did not reveal any significant difference between fat and lean chickens.
在经过基因选择的肥胖和瘦型鸡中研究了灌注胰腺的胰岛素释放情况。先前描述的鸡胰腺对葡萄糖不敏感的情况不能被5或10 mM的D -甘油醛克服，这表明这种抗性与磷酸丙糖步骤之前的葡萄糖代谢无关。在42 mM时，葡萄糖诱导了双相胰岛素释放，这是特异性的，因为42 mM的甘露醇不会引发胰岛素释放。精氨酸（10 mM）或乙酰胆碱（0.1 - 1 microM）本身不会引起胰岛素释放，但在低的非刺激葡萄糖浓度（14 mM）存在时会产生双相胰岛素释放；这种作用是协同的。与体内观察到的葡萄糖耐量试验相反，来自肥胖品系鸡的胰腺在第一阶段对葡萄糖或葡萄糖加精氨酸的反应中释放的胰岛素明显较少。这种缺陷的意义有待进一步阐明。另一方面，乙酰胆碱是一种更强效的促分泌剂，在肥胖和瘦型鸡之间未显示出任何显著差异。
