Jokura Y, Urano A
Gen Comp Endocrinol. 1986 Apr;62(1):80-8. doi: 10.1016/0016-6480(86)90096-1.
Extrahypothalamic projection of luteinizing hormone-releasing hormone (LHRH) fibers in the brain of the toad (Bufo japonicus) was examined immunohistochemically by the avidin-biotin-peroxidase complex (ABC) method. Immunoreactive LHRH perikarya are localized in the nuclei medialis septi and of the diagonal band of Broca. A part of the LHRH fibers are sent anteriad to the medial and dorsal pallia. Some fibers reach the olfactory bulb. Dorsocaudally, LHRH neurons in the medial septum project their fibers to the deep layers of the optic tectum and the posterior mesencephalon including the nucleus pretrigeminalis, which is considered to be a generator of mate calling behavior, via the habenular and posterior thalamic regions. In addition, LHRH fibers which run caudad through the dorsal infundibular region and then the mesencephalic reticular formation were widely distributed in both the gray and the white matter of the medulla oblongata. These findings suggest that LHRH acts as a neurotransmitter or a neuromodulator in the various neuronal circuitries for reproductive behavior in the central nervous system, because LHRH has been considered to be related to amphibian seasonal breeding, and many regions where the immunoreactive LHRH fibers were observed are the loci concerned with mating behavior.
采用抗生物素蛋白-生物素-过氧化物酶复合物（ABC）法，通过免疫组织化学方法研究了蟾蜍（日本蟾蜍）脑中促黄体生成激素释放激素（LHRH）纤维的下丘脑外投射。免疫反应性LHRH核周体位于内侧隔核和布罗卡斜带核。一部分LHRH纤维向前内侧和背侧大脑皮质发出。一些纤维到达嗅球。在内侧隔核中，LHRH神经元的纤维通过缰核和丘脑后区向背尾侧投射到视顶盖深层和中脑后部，包括三叉神经前核，该核被认为是求偶行为的产生部位。此外，LHRH纤维沿尾侧穿过漏斗背侧区，然后通过中脑网状结构，广泛分布于延髓的灰质和白质中。这些发现表明，LHRH在中枢神经系统中参与生殖行为的各种神经回路中作为神经递质或神经调节剂发挥作用，因为LHRH被认为与两栖动物的季节性繁殖有关，并且观察到免疫反应性LHRH纤维的许多区域是与交配行为相关的位点。
