Department of Pharmacy and Medicine, Tra Vinh University, Tra Vinh City, Vietnam.
Institute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam.
Curr Drug Deliv. 2022 Aug 6;19(9):966-979. doi: 10.2174/1567201819666220401095923.
Multi-drug nanosystem has been employed in several therapeutic models due to the synergistic effect of the drugs and/or bioactive compounds, which help in tumor targeting and limit the usual side effects of chemotherapy.
In this research, we developed the amphiphilic Heparin-poloxamer P403 (HSP) nanogel that could load curcumin (CUR) and Paclitaxel (PTX) through the hydrophobic core of Poloxamer P403. The features of HSP nanogel were assessed through Fourier-transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), differential light scattering (DLS), and critical micelle concentration (CMC). Nanogel and its dual drug-loaded platform showed high stability and spherical morphology.
The drug release profile indicated fast release at pH 5.5, suggesting effective drug distribution at the tumor site. In vitro research confirms lower cytotoxicity of HSP@CUR@PTX compared to free PTX and higher inhibition effect with MCF-7 than HSP@PTX. These results support the synergism between PTX and CUR.
HSP@CUR@PTX suggests a prominent strategy for achieving the synergistic effect of PTX and CUR to circumvent undesirable effects in breast cancer treatment.
多药纳米系统由于药物和/或生物活性化合物的协同作用，已被应用于几种治疗模型中，这有助于肿瘤靶向，并限制化疗的常见副作用。
在这项研究中，我们开发了两亲性肝素-泊洛沙姆 P403（HSP）纳米凝胶，通过泊洛沙姆 P403 的疏水核心可以装载姜黄素（CUR）和紫杉醇（PTX）。通过傅里叶变换红外光谱（FT-IR）、透射电子显微镜（TEM）、差示光散射（DLS）和临界胶束浓度（CMC）评估 HSP 纳米凝胶的特性。纳米凝胶及其双载药平台表现出高稳定性和球形形态。
药物释放曲线表明在 pH5.5 时快速释放，表明药物在肿瘤部位有效分布。体外研究证实 HSP@CUR@PTX 比游离 PTX 的细胞毒性更低，对 MCF-7 的抑制作用高于 HSP@PTX。这些结果支持 PTX 和 CUR 之间的协同作用。
HSP@CUR@PTX 为实现 PTX 和 CUR 的协同作用提供了一种有前途的策略，以避免乳腺癌治疗中的不良影响。
