Department of Pathology, Renaissance School of Medicine, Stony Brook University, Stony Brook, NY, United States.
Department of Pathology, Renaissance School of Medicine, Stony Brook University, Stony Brook, NY, United States; Stony Brook Cancer Center, Stony Brook, NY, United States.
Methods Cell Biol. 2022;168:1-17. doi: 10.1016/bs.mcb.2021.12.032. Epub 2022 Jan 28.
Inflammatory bowel diseases (IBD) involve repetitive bouts of inflammation in the intestinal tract and can result in severe morbidity for patients. Moreover, long-standing IBD increases the risk for developing intestinal neoplasia. Although several factors including immune cell activity, microbiota and diet have been implicated in IBD pathogenesis, it is still considered a disease of idiopathic origin. Therefore, much work is needed to identify the critical mediators in IBD onset, severity and response to treatment. Mouse models are useful for identifying factors that contribute to IBD and the efficacy of therapy, which can then be tested in humans. There are currently multiple IBD models including the use of chemical induction, genetic manipulation and modulation of the immune response. The T cell transfer colitis model provides a quality mimic of human IBD that is T cell driven and results in inflammation in both the ileum and colon. Here, we have provided a detailed step-by-step protocol to induce inflammation and assess disease severity using this model. Such a detailed methodologic description will help to increase its utilization to advance IBD research.
炎症性肠病（IBD）涉及肠道的反复炎症发作，可导致患者严重发病。此外，长期的 IBD 会增加发生肠道肿瘤的风险。尽管包括免疫细胞活性、微生物组和饮食在内的多种因素都与 IBD 的发病机制有关，但它仍被认为是一种特发性疾病。因此，需要开展大量工作来确定 IBD 发病、严重程度和治疗反应的关键介质。小鼠模型有助于确定导致 IBD 发生的因素以及治疗的效果，然后可以在人体中进行测试。目前有多种 IBD 模型，包括使用化学诱导、遗传操作和免疫反应调节。T 细胞转移结肠炎模型提供了一种模拟人类 IBD 的优质模型，该模型由 T 细胞驱动，可导致回肠和结肠同时发生炎症。在这里，我们提供了一个详细的分步协议，使用该模型诱导炎症并评估疾病严重程度。这样详细的方法描述将有助于增加其在推进 IBD 研究中的应用。
