Department of Microbiology and Immunology, Stony Brook University, Stony Brook, NY, United States.
Department of Microbiology and Immunology, Stony Brook University, Stony Brook, NY, United States.
Methods Cell Biol. 2022;168:315-327. doi: 10.1016/bs.mcb.2021.12.022. Epub 2022 Jan 21.
The human fungal pathogen Candida albicans (C. albicans) causes invasive candidiasis, characterized by fatal organ failure due to disseminated fungal growth and inflammatory damage. To better understand fungal pathogenicity mechanisms and host protective responses, a murine model of invasive candidiasis has been developed in which C. albicans is administered systemically via intravenous injection. In this infection model, all major tissues are seeded within 0-4h. Of all the peripheral organs, the kidneys provide the most favorable niches for fungal proliferation and the morphogenetic switch to a hyphal state. As a consequence, the kidneys are a focal point for analyzing many of the genetic and immunological factors that underlie disease progression. Herein, we describe a number of well-established techniques that allow investigation into specific mechanisms that impact host-pathogen interactions.
人源真菌病原体白色念珠菌（Candida albicans，C. albicans）会引起侵袭性念珠菌病，其特征是由于真菌扩散生长和炎症损伤而导致致命的器官衰竭。为了更好地理解真菌致病性机制和宿主保护反应，已经开发了一种侵袭性念珠菌病的小鼠模型，其中通过静脉内注射系统地给予白色念珠菌。在这种感染模型中，所有主要组织在 0-4 小时内被定植。在所有外周器官中，肾脏为真菌增殖和形态发生切换到菌丝状态提供了最有利的小生境。因此，肾脏是分析许多遗传和免疫因素的重点，这些因素是疾病进展的基础。在此，我们描述了许多已建立的技术，这些技术可用于研究影响宿主-病原体相互作用的特定机制。
