Departamento de Zootecnia, Setor de Piscicultura, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, 13418-900 Piracicaba, SP, Brazil.
Department of Biology, Memorial University of Newfoundland, St. John's, NL, A1B 3X9, Canada.
Comp Biochem Physiol A Mol Integr Physiol. 2022 Jun;268:111208. doi: 10.1016/j.cbpa.2022.111208. Epub 2022 Mar 31.
Dourado (Salminus brasiliensis) is a large carnivorous fish with high commercial value for which sustainable aquaculture relies on the substitution of expensive dietary animal protein sources in aquafeeds, in particular fish meal (FM), by cheaper plant protein, such as soy protein concentrate (SPC). This study aimed at evaluating feed intake and gene expression of appetite- regulating hormones [orexin, cocaine and amphetamine regulated transcript (CART), leptin, cholecystokinin (CCK) and peptide YY (PYY)] in the intestine, pyloric caeca and hypothalamus of juvenile dourado fed diets containing graded levels of SPC and FM as dietary protein sources for a period of three weeks. Increasing dietary plant protein contents reduced daily feed consumption and the expressions of the anorexigenic hormone CCK in the anterior intestine and in pyloric caeca and PYY in pyloric caeca. No changes were detected in the hypothalamic expression of appetite-regulating hormones, suggesting that gastrointestinal hormones are more involved in the decrease in feeding induced by plant protein diets than central appetite-regulating systems.
金臀丽鱼（Salminus brasiliensis）是一种大型肉食性鱼类，商业价值很高，其可持续水产养殖依赖于在水产饲料中用更便宜的植物蛋白替代昂贵的动物蛋白饲料，特别是鱼粉（FM），如大豆浓缩蛋白（SPC）。本研究旨在评估在 3 周的时间内，用含有不同水平 SPC 和 FM 的饲料喂养金臀丽鱼幼鱼后，其肠道、幽门盲囊和下丘脑的摄食调节激素[食欲肽、可卡因和安非他命调节转录物（CART）、瘦素、胆囊收缩素（CCK）和肽 YY（PYY）]的摄食量和基因表达。随着日粮植物蛋白含量的增加，每日采食量和前肠及幽门盲囊的厌食激素 CCK 以及幽门盲囊的 PYY 表达降低。在摄食调节激素的下丘脑表达中未检测到变化，这表明胃肠道激素在植物蛋白日粮引起的摄食减少中比中枢摄食调节系统更起作用。
