微生物电合成中同时利用二氧化碳和乙醇高效生产中链脂肪酸。
Efficient production of medium chain fatty acids in microbial electrosynthesis with simultaneous bio-utilization of carbon dioxide and ethanol.
机构信息
Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
出版信息
Bioresour Technol. 2022 May;352:127101. doi: 10.1016/j.biortech.2022.127101. Epub 2022 Mar 31.
Microbial electrosynthesis (MES) is a promising technology for chemicals production driven by renewable energy. However, how the medium chain fatty acids (MCFAs) production in MES is affected by the method of chain elongation is not clear, and no direct evidence is provided yet for a simultaneous bio-utilization of CO and ethanol. In this study, different methods of chain elongation in MES reactors were investigated. During in-situ chain elongation, a maximum caproate concentration of 11.9 ± 0.6 g L was achieved, while the C6 specificity (56.4% ± 0.5%) was much lower than that of ex-situ chain elongation (78.7% ± 1.5%). Carbon distribution and reduction degree balance indicated a simultaneous bio-utilization of CO and ethanol, and it was validated by the isotope tracer technique. MCFAs-forming microbes, acetogens, and electrochemically active microorganisms were enriched. This study provides fundamental insights relevant to the carbon and electron fluxes driven by electricity.
微生物电解合成（MES）是一种有前途的技术，可利用可再生能源来生产化学品。然而，MES 中中链脂肪酸（MCFAs）的产生受链伸长方法的影响尚不清楚，而且目前还没有关于 CO 和乙醇同时生物利用的直接证据。在本研究中，考察了 MES 反应器中不同的链伸长方法。在原位链伸长过程中，获得了 11.9 ± 0.6 g/L 的最大己酸浓度，而 C6 特异性（56.4% ± 0.5%）明显低于异位链伸长（78.7% ± 1.5%）。碳分布和还原程度平衡表明 CO 和乙醇可同时进行生物利用，并通过同位素示踪技术得到验证。富集了形成 MCFAs 的微生物、乙酰菌和电化学活性微生物。本研究为电驱动的碳和电子通量提供了基础见解。
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