Meek W D, Davis W L
In Vitro Cell Dev Biol. 1986 Dec;22(12):716-24. doi: 10.1007/BF02621089.
Morphological studies of the WISH cell line reveal an epithelioid cell type with some characteristics of both the original human amnion epithelium and a transformed state. WISH cells have a cytoplasm filled with microtubules; however, actin filament bundles are few, with actin localized at areas of cell contact and arranged diffusely through the cytoplasm, as viewed by indirect immunofluorescence. Fingerlike projections or short filopodia are observed connecting cells that grow in a closely apposed monolayer. Other surface features, as viewed by scanning electron microscopy, include microvilli and blebs. Transmission electron microscopy shows that WISH cultures consist of light or dark cells with organelles that include lipid droplets, abundant free ribosomes, tubular mitochondria, lysosomes, annulate lamellae, rough endoplasmic reticulum, 6-nm microfilaments, 10-nm intermediate filaments, and microtubules. Pleomorphic nuclei with multiple nucleoli and fibrillar nuclear bodies are common. Desmosomes and subsurface confronting cisternae connect cells. To our knowledge, these structural studies are the first to describe WISH and lead to subsequent investigation of the cell surface phenomenon of blebbing and surface charge in WISH and another human cell line.
对WISH细胞系的形态学研究显示，其为上皮样细胞类型，兼具原始人羊膜上皮和转化状态的一些特征。WISH细胞的细胞质中充满微管；然而，肌动蛋白丝束较少，通过间接免疫荧光观察，肌动蛋白定位于细胞接触区域，并在细胞质中呈弥散分布。在紧密并列生长的单层细胞中，可观察到指状突起或短丝状伪足连接细胞。通过扫描电子显微镜观察到的其他表面特征包括微绒毛和泡状突起。透射电子显微镜显示，WISH培养物由浅色或深色细胞组成，细胞器包括脂滴、丰富的游离核糖体、管状线粒体、溶酶体、环状片层、粗面内质网、6纳米微丝、10纳米中间丝和微管。具有多个核仁及纤维状核体的多形核较为常见。桥粒和表面下相对池连接细胞。据我们所知，这些结构研究首次描述了WISH细胞，并引发了对WISH细胞系和另一种人类细胞系中泡状突起和表面电荷的细胞表面现象的后续研究。
