Beijing Clinical Research Institute, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China.
Biotechniques. 2022 May;72(5):219-224. doi: 10.2144/btn-2022-0024. Epub 2022 Apr 4.
Gene manipulation in non-adhesive cells, especially lymphocytes, was difficult due to their low efficiency and high toxicity. Electroporation was reported as a highly efficient method for human and mouse lymphocytes. However, this method requires expensive equipment and causes severe cell damage. Here, the authors present a simple and efficient method to deliver siRNA into lymphocytes with high efficiency and cell viability. This nanomaterials-based transfection reagent was simple and cost-effective and can perform multiple transfections, which further increase the overall efficiency. This method should be applicable for many cell lines and can be used to decipher gene functions of lymphocytes.
由于非黏附细胞（尤其是淋巴细胞）的基因转导效率低、毒性高，因此其基因操作较为困难。电穿孔被报道为一种高效的人源和鼠源淋巴细胞的基因转导方法。然而，这种方法需要昂贵的设备并且会造成严重的细胞损伤。在这里，作者提出了一种简单高效的方法，可将 siRNA 高效且高细胞活力地递送至淋巴细胞。这种基于纳米材料的转染试剂简单且具有成本效益，可进行多次转染，从而进一步提高整体效率。该方法应该适用于许多细胞系，可用于解析淋巴细胞的基因功能。
