Umeda M, Ishimori Y, Yoshikawa K, Takada M, Yasuda T
J Immunol Methods. 1986 Dec 4;95(1):15-21. doi: 10.1016/0022-1759(86)90312-1.
A complement-dependent liposome immune lysis assay (LILA) using carboxyfluorescein (CF)-entrapped liposomes bearing antibody was developed to measure C-reactive protein (CRP) antigen as a model of protein antigens in human sera. Goat anti-CRP antibody was covalently coupled to liposomes, and a specific lysis of the liposomes could be observed when the liposomes were incubated with both rabbit anti-CRP antibody (secondary antibody) and CRP antigen in sera in the presence of guinea pig complement. In this assay system, so-called sandwich assay, CRP (a multivalent antigen) bound to the liposomes bearing anti-CRP antibody and subsequently secondary antibody, which activated complement efficiently. The amount of CF released by a complement-dependent liposome immune lysis was proportional to CRP concentrations. This sandwich assay was simple, fast, highly sensitive, and covered the ranges 10-300 ng of CRP/ml in a homogeneous mode, that is, one where no separation step was employed. The results correlated well with those obtained by single radial immunodiffusion and enzyme immunoassay. This assay system would be applicable to the measurement of other protein antigens.
开发了一种基于补体的脂质体免疫裂解测定法（LILA），该方法使用包载抗体且 entrapped 羧基荧光素（CF）的脂质体来测定人血清中作为蛋白质抗原模型的 C 反应蛋白（CRP）抗原。将山羊抗 CRP 抗体共价偶联到脂质体上，当脂质体与兔抗 CRP 抗体（二抗）和血清中的 CRP 抗原在豚鼠补体存在的情况下孵育时，可观察到脂质体的特异性裂解。在该测定系统（即所谓的夹心测定法）中，CRP（一种多价抗原）与包载抗 CRP 抗体的脂质体结合，随后与二抗结合，从而有效激活补体。补体依赖性脂质体免疫裂解释放的 CF 量与 CRP 浓度成正比。这种夹心测定法简单、快速、高度灵敏，并且以均相模式覆盖了 10 - 300 ng CRP/ml 的范围，即无需分离步骤的模式。结果与通过单向放射免疫扩散和酶免疫测定法获得的结果相关性良好。该测定系统可应用于其他蛋白质抗原的测量。 （注：原文中“carboxyfluorescein (CF)-entrapped liposomes”中“entrapped”翻译为“包载的”，但感觉此处表述不太准确，可能原文有误，若按字面意思应是“包裹羧基荧光素（CF）的脂质体”，不过整体翻译还是按原文进行了。）
