Villaverde A, Guerrero R, Barbé J
J Gen Virol. 1986 Nov;67 ( Pt 11):2561-4. doi: 10.1099/0022-1317-67-11-2561.
Infection with u.v.-inactivated P22 bacteriophage at multiplicities higher than 30 caused a decrease in Salmonella typhimurium viability without cell lysis. Neither the action of the endoglycosidase of the P22 virion on the lipopolysaccharide of S. typhimurium nor the concomitant release of cell wall components was responsible for m.o.i.-dependent cell death. Using both free P22 tails and u.v.-inactivated P22, we have shown that p9 tail protein activity has no effect either on the integrity of host cells or on cell viability. Our results show that cell death is due to the injection of the u.v.-inactivated P22 DNA.
用紫外线灭活的P22噬菌体以高于30的感染复数感染，会导致鼠伤寒沙门氏菌的存活率下降但不会导致细胞裂解。P22病毒粒子的内切糖苷酶对鼠伤寒沙门氏菌脂多糖的作用以及细胞壁成分的同时释放，均与感染复数依赖性细胞死亡无关。使用游离的P22尾部和紫外线灭活的P22，我们已经表明p9尾部蛋白活性对宿主细胞的完整性或细胞活力均无影响。我们的结果表明，细胞死亡是由于紫外线灭活的P22 DNA的注入。
