Schumann W, Lindenblatt E, Bade E G
J Virol. 1976 Oct;20(1):334-8. doi: 10.1128/JVI.20.1.334-338.1976.
Crude extracts of Salmonella typhimurium lysogenic for phages P22 or L contain proteins that specifically retain phage DNA on nitrocellulose filters. Three DNA-binding activities were found after infection with P22. One is P22 specific, accounts for the largest proportion of DNA-binding proteins, and corresponds most likely to the c2 repressor. An early transient binding activity measured with both P22 and L DNA was found to be directly related to the expression of genes c1 and c3. A third, late binding activity for P22 and L DNA is related to phage production.
携带噬菌体P22或L的鼠伤寒沙门氏菌的粗提物含有能将噬菌体DNA特异性保留在硝酸纤维素滤膜上的蛋白质。感染P22后发现了三种DNA结合活性。一种是P22特异性的,占DNA结合蛋白的最大比例,最有可能对应于c2阻遏物。用P22和L DNA均检测到的一种早期瞬时结合活性与基因c1和c3的表达直接相关。P22和L DNA的第三种晚期结合活性与噬菌体产生有关。