Department of Microbiology and Immunology, Witebsky Center for Microbial Pathogenesis and Immunology, Jacobs School of Medicine and Biomedical Sciences, State University of New York (SUNY), University at Buffalo, Buffalo, NY, United States.
Front Cell Infect Microbiol. 2022 Mar 17;12:858317. doi: 10.3389/fcimb.2022.858317. eCollection 2022.
Long non-coding RNAs (lncRNAs) are highly expressed and can modulate multiple cellular processes including transcription, splicing, translation, and many diverse signaling events. LncRNAs can act as sponges for miRNAs, RNA and DNA binding proteins, functioning as competitive endogenous RNAs. The contribution of lncRNAs to microbial pathogenesis is largely neglected in eukaryotic pathogens despite the abundance of RNA sequencing datasets encompassing conditions of stress, gene deletions and conditions that mimic the host environment. The human fungal pathogen encodes 6975 (84%) protein-coding and 1359 (16%) non-protein-coding RNAs, of which 1182 (14.2%) are lncRNAs defined by a threshold of greater than 200 nucleotides in length. Here, we discuss the current state of knowledge in lncRNA biology. Utilizing existing RNA seq datasets, we examine trends in lncRNA expression and discuss potential implications for pathogenesis.
长非编码 RNA（lncRNA）表达丰度高，能够调节多种细胞过程，包括转录、剪接、翻译和许多不同的信号事件。lncRNA 可以作为 miRNA 的海绵，与 RNA 和 DNA 结合蛋白结合，充当竞争性内源性 RNA。尽管 RNA 测序数据集涵盖了应激、基因缺失和模拟宿主环境的条件等情况，但真核病原体中 lncRNA 对微生物发病机制的贡献在很大程度上被忽视了。人类真菌病原体 编码了 6975 个（84%）蛋白编码和 1359 个（16%）非蛋白编码 RNA，其中 1182 个（14.2%）是长度大于 200 个核苷酸的长非编码 RNA。在这里，我们讨论了 lncRNA 生物学的当前知识状态。利用现有的 RNA seq 数据集，我们研究了 lncRNA 表达的趋势，并讨论了其对发病机制的潜在影响。
