Consistency of protein patterns in Candida albicans during hyphal septum and branch formation.

Candida albicans was grown in its hyphal form in five different media and in the presence of two concentrations of ketoconazole, and in its yeast form in one medium. Polyacrylamide gel electrophoresis patterns of proteins obtained by disruption of the fungal cells at different times around the stages of septum formation and branch initiation revealed no differences in intracellular protein composition related to growth conditions or time. Autoradiography of proteins labelled with [35S1]-methionine similarly revealed no differences in protein composition related to growth conditions or time. These findings suggest either that septum formation and branch initiation proceed independently of synthesis of new proteins or that proteins related to these processes are synthesized too transiently or in amounts too small to be detected under the conditions of the experiments.