Gow N A, Henderson G, Gooday G W
Microbios. 1986;47(191):97-105.
The cytology of nuclear division and septation in the yeast and hyphal phases of Candida albicans growing at 37 degrees C has been studied by fluorescence microscopy after staining of specimens with 4'6-diaminido-2-phenylindole (DAPI) and Calcofluor. Yeast and hyphal cells replicated their nuclei at about 18 min after the emergence of a bud or germ-tube. The site of nuclear division coincided with the future location of the septum in both forms. This occurred at the junction of the bud and parent yeast cell or 6.0 micron from the parent yeast in germ tubes which were formed in medium containing serum. The filamentous forms of a range of clinical and laboratory strains grown in a variety of germ tube-inducing media were all extensively vacuolated. Germ tube extension in all of these media was linear. It is suggested that there is little biosynthesis of cytoplasm during the initial stages of germ tube growth in this organism and that this accounts for the development of the large vacuoles and the linear growth kinetics.
利用4'6-二脒基-2-苯基吲哚（DAPI）和荧光增白剂对标本进行染色后，通过荧光显微镜研究了白色念珠菌在37℃下生长的酵母期和菌丝期的核分裂及隔膜形成的细胞学特征。酵母细胞和菌丝细胞在芽或芽管出现后约18分钟时进行细胞核复制。两种形态下核分裂的位置都与隔膜未来的位置一致。这发生在芽与母酵母细胞的交界处，或者在含血清培养基中形成的芽管中距离母酵母6.0微米处。在各种芽管诱导培养基中生长的一系列临床和实验室菌株的丝状形态都有大量液泡。在所有这些培养基中芽管的延伸都是线性的。有人提出，在该生物体芽管生长的初始阶段，细胞质的生物合成很少，这就解释了大液泡的形成和线性生长动力学。
