针对ras基因产物的抗体可抑制腺苷酸环化酶，并加速非洲爪蟾卵母细胞中孕酮诱导的细胞分裂。
Antibodies to the ras gene product inhibit adenylate cyclase and accelerate progesterone-induced cell division in Xenopus laevis oocytes.
作者信息
Sadler S E, Schechter A L, Tabin C J, Maller J L
出版信息
Mol Cell Biol. 1986 Feb;6(2):719-22. doi: 10.1128/mcb.6.2.719-722.1986.
Abstract
Microinjection of monoclonal antibodies (lines 238, 172, and 259) directed against the ras gene product, p21, into Xenopus laevis oocytes accelerated progesterone-induced germinal vesicle breakdown. Antibody 238 had the greatest effect on the acceleration of progesterone-induced oocyte maturation, and this effect was correlated with in vitro inhibition of adenylate cyclase (EC 4.6.1.1) activity in a concentration-dependent manner. Inhibition of adenylate cyclase by antibody 238 was also measured in membranes prepared from oocytes pretreated with either cholera toxin or pertussis toxin. These results suggest a role for the ras gene product in the regulation of vertebrate cell adenylate cyclase activity.
摘要
将针对ras基因产物p21的单克隆抗体（238、172和259株系）显微注射到非洲爪蟾卵母细胞中，加速了孕酮诱导的生发泡破裂。抗体238对孕酮诱导的卵母细胞成熟加速作用最大，且该作用与体外对腺苷酸环化酶（EC 4.6.1.1）活性的抑制呈浓度依赖性相关。在由用霍乱毒素或百日咳毒素预处理的卵母细胞制备的膜中也检测到了抗体238对腺苷酸环化酶的抑制作用。这些结果表明ras基因产物在脊椎动物细胞腺苷酸环化酶活性调节中发挥作用。
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本文引用的文献
1
Progesterone inhibits adenylate cyclase in Xenopus oocytes. Action on the guanine nucleotide regulatory protein.孕酮抑制非洲爪蟾卵母细胞中的腺苷酸环化酶。对鸟嘌呤核苷酸调节蛋白的作用。
J Biol Chem. 1981 Jun 25;256(12):6368-73.
2
Progesterone inhibits membrane-bound adenylate cyclase in Xenopus laevis oocytes.孕酮抑制非洲爪蟾卵母细胞中的膜结合腺苷酸环化酶。
Nature. 1981 Jul 16;292(5820):255-7. doi: 10.1038/292255a0.
3
Activation of ras genes in human tumors does not affect localization, modification, or nucleotide binding properties of p21.人类肿瘤中ras基因的激活并不影响p21的定位、修饰或核苷酸结合特性。
Cell. 1984 May;37(1):151-8. doi: 10.1016/0092-8674(84)90310-6.
4
5
Does the guanine nucleotide regulatory protein Ni mediate progesterone inhibition of Xenopus oocyte adenylate cyclase?鸟嘌呤核苷酸调节蛋白Ni是否介导孕酮对非洲爪蟾卵母细胞腺苷酸环化酶的抑制作用？
EMBO J. 1984 Nov;3(11):2653-7. doi: 10.1002/j.1460-2075.1984.tb02189.x.
6
Homologies between signal transducing G proteins and ras gene products.信号转导G蛋白与ras基因产物之间的同源性。
Science. 1984 Nov 16;226(4676):860-2. doi: 10.1126/science.6436980.
7
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