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在酿酒酵母体内，起始因子2的α亚基会被磷酸化。

The alpha subunit of initiation factor 2 is phosphorylated in vivo in the yeast Saccharomyces cerevisiae.

作者信息

Romero D P, Dahlberg A E

出版信息

Mol Cell Biol. 1986 Apr;6(4):1044-9. doi: 10.1128/mcb.6.4.1044-1049.1986.

DOI:10.1128/mcb.6.4.1044-1049.1986
PMID:3537696
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC367613/
Abstract

The phosphorylation state of the alpha subunit of initiation factor 2 (eIF-2 alpha) in Saccharomyces cerevisiae has been determined by two-dimensional gel electrophoresis and autoradiography of lysates from cultures grown under a variety of conditions. The alpha subunit was maintained in a phosphorylated state during logarithmic growth on fermentable and nonfermentable carbon sources, during starvation for an essential amino acid, during heat shock, during stationary phase, and during sporulation. Only when cells were starved for a carbon source for 2 h in 1 M sorbitol was eIF-2 alpha isolated in the nonphosphorylated state. This is in contrast with the studies in rabbit reticulocyte lysates, in which arrested protein synthesis was correlated with a relative increase in the extent of phosphorylation of eIF-2 alpha.

摘要

通过二维凝胶电泳和放射自显影技术，对在各种条件下培养的酿酒酵母细胞裂解物中的起始因子2（eIF - 2α）α亚基的磷酸化状态进行了测定。在对数生长期，当细胞利用可发酵和不可发酵碳源生长时、在必需氨基酸饥饿期间、热休克期间、稳定期以及孢子形成期间，α亚基均保持磷酸化状态。只有当细胞在1 M山梨醇中缺乏碳源饥饿2小时时，eIF - 2α才以非磷酸化状态被分离出来。这与在兔网织红细胞裂解物中的研究结果相反，在兔网织红细胞裂解物中，蛋白质合成停滞与eIF - 2α磷酸化程度的相对增加相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38eb/367613/5c3cb0c5a4be/molcellb00088-0089-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38eb/367613/3e6abbcb94e1/molcellb00088-0087-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38eb/367613/1bd06749e287/molcellb00088-0088-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38eb/367613/bc591dcb42af/molcellb00088-0088-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38eb/367613/5eb243cf4dfd/molcellb00088-0089-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38eb/367613/83076a22c2db/molcellb00088-0089-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38eb/367613/5c3cb0c5a4be/molcellb00088-0089-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38eb/367613/3e6abbcb94e1/molcellb00088-0087-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38eb/367613/1bd06749e287/molcellb00088-0088-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38eb/367613/bc591dcb42af/molcellb00088-0088-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38eb/367613/5eb243cf4dfd/molcellb00088-0089-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38eb/367613/83076a22c2db/molcellb00088-0089-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38eb/367613/5c3cb0c5a4be/molcellb00088-0089-c.jpg

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The alpha subunit of initiation factor 2 is phosphorylated in vivo in the yeast Saccharomyces cerevisiae.在酿酒酵母体内，起始因子2的α亚基会被磷酸化。
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引用本文的文献

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Casein kinase II mediates multiple phosphorylation of Saccharomyces cerevisiae eIF-2 alpha (encoded by SUI2), which is required for optimal eIF-2 function in S. cerevisiae.酪蛋白激酶II介导酿酒酵母eIF-2α（由SUI2编码）的多重磷酸化，这是酿酒酵母中eIF-2发挥最佳功能所必需的。
Mol Cell Biol. 1994 Aug;14(8):5139-53. doi: 10.1128/mcb.14.8.5139-5153.1994.
2
Yeast translation initiation suppressor sui2 encodes the alpha subunit of eukaryotic initiation factor 2 and shares sequence identity with the human alpha subunit.酵母翻译起始抑制因子sui2编码真核起始因子2的α亚基，并与人α亚基具有序列同源性。
Proc Natl Acad Sci U S A. 1989 Apr;86(8):2784-8. doi: 10.1073/pnas.86.8.2784.

本文引用的文献

1
Phosphorylation inhibits guanine nucleotide exchange on eukaryotic initiation factor 2.磷酸化抑制真核生物起始因子2上的鸟嘌呤核苷酸交换。
Nature. 1982 Mar 4;296(5852):93-5. doi: 10.1038/296093a0.
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Isolation of eukaryotic initiation factor 2 from yeast Saccharomyces cerevisiae.从酿酒酵母中分离真核生物起始因子2
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Messenger ribonucleic acid and protein metabolism during sporulation of Saccharomyces cerevisiae.酿酒酵母孢子形成过程中的信使核糖核酸与蛋白质代谢
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Regulation of protein synthesis during heat shock.热休克期间蛋白质合成的调控。
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The effect of serum, EGF, PGF2 alpha and insulin on S6 phosphorylation and the initiation of protein and DNA synthesis.血清、表皮生长因子、前列腺素F2α和胰岛素对S6磷酸化以及蛋白质和DNA合成起始的影响。
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2B or not 2B: regulation of the catalytic utilization of eIF-2.2B还是非2B：真核生物翻译起始因子2（eIF-2）催化利用的调控
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Mechanism of interferon action. Increased phosphorylation of protein synthesis initiation factor eIF-2 alpha in interferon-treated, reovirus-infected mouse L929 fibroblasts in vitro and in vivo.
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Changing patterns of gene expression during sporulation in yeast.酵母孢子形成过程中基因表达模式的变化。
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Heat shock-induced translational alterations in HeLa cells. Initiation factor modifications and the inhibition of translation.热休克诱导的HeLa细胞翻译改变。起始因子修饰与翻译抑制。
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Regulation of protein synthesis initiation in eucaryotes.真核生物中蛋白质合成起始的调控。
Arch Biochem Biophys. 1983 Jun;223(2):325-49. doi: 10.1016/0003-9861(83)90598-2.