Laboratory of Aquaculture Genetics and Genomics, Division of Marine Life Science, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, 041-8611, Japan.
Mar Biotechnol (NY). 2022 Apr;24(2):393-407. doi: 10.1007/s10126-022-10121-3. Epub 2022 Apr 4.
Many organisms are subjected to a daily cycle of light and darkness, which significantly influences metabolic and physiological processes. In the present study, Neopyropia yezoensis, one of the major cultivated seaweeds used in "nori," was harvested in the morning and evening during light/dark treatments to investigate daily changes in gene expression using RNA-sequencing. A high abundance of transcripts in the morning includes the genes associated with carbon-nitrogen assimilations, polyunsaturated fatty acid, and starch synthesis. In contrast, the upregulation of a subset of the genes associated with the pentose phosphate pathway, cell cycle, and DNA replication at evening is necessary for the tight control of light-sensitive processes, such as DNA replication. Additionally, a high abundance of transcripts at dusk encoding asparaginase and glutamate dehydrogenase imply that regulation of asparagine catabolism and tricarboxylic acid cycle possibly contributes to supply nitrogen and carbon, respectively, for growth during the dark. In addition, genes encoding cryptochrome/photolyase family and histone modification proteins were identified as potential key players for regulating diurnal rhythmic genes.
许多生物都受到光照和黑暗的日常循环的影响，这显著影响代谢和生理过程。在本研究中，我们在光照/黑暗处理期间早晚收获了一种主要的养殖海藻——日本紫菜，以使用 RNA 测序来研究基因表达的日常变化。大量的转录物在早晨出现，包括与碳氮同化、多不饱和脂肪酸和淀粉合成相关的基因。相比之下，傍晚与戊糖磷酸途径、细胞周期和 DNA 复制相关的一组基因的上调对于严格控制光敏感过程（如 DNA 复制）是必要的。此外，黄昏时大量出现的编码天冬酰胺酶和谷氨酸脱氢酶的转录物表明，天冬酰胺分解代谢和三羧酸循环的调节可能分别为黑暗期间的生长提供氮和碳。此外，鉴定出编码隐色体/光解酶家族和组蛋白修饰蛋白的基因作为调节昼夜节律基因的潜在关键因子。
