Molecular and Cellular Biology Department, University of Guelph, Guelph, ON, N1G 2W1, Canada; Canadian Proteomics and Artificial Intelligence Research and Training Consortium, Canada.
Molecular and Cellular Biology Department, University of Guelph, Guelph, ON, N1G 2W1, Canada.
Curr Opin Chem Biol. 2022 Jun;68:102133. doi: 10.1016/j.cbpa.2022.102133. Epub 2022 Apr 1.
Molecular pharming relies on the integration of foreign genes into a plant system for production of the desired recombinant protein. The speed, scalability, and lack of contaminating human pathogens highlights plants as an enticing and feasible system to produce diverse protein-based products, including vaccines, antibodies, and enzymes. However, limitations of expression levels, host defense responses, and production irregularities underscore distinct areas for improvement within the molecular pharming pipeline. Within the past five years, mass spectrometry-based proteomics has begun to address these critical areas and show promise in advancing our understanding of the complex biological systems driving molecular pharming. Further, opportunities to leverage comprehensive proteome profiling have surfaced to meet good manufacturing practice regulations and move biopharmaceuticals derived from plants into mainstream production.
分子农业依赖于将外源基因整合到植物系统中，以生产所需的重组蛋白。植物具有生产各种基于蛋白质的产品（包括疫苗、抗体和酶）的速度快、可扩展性强且不存在污染人类病原体的特点，这使其成为一种诱人且可行的系统。然而，表达水平、宿主防御反应和生产不规则性的限制突显了分子农业管道中需要改进的明显领域。在过去的五年中，基于质谱的蛋白质组学已经开始解决这些关键领域，并有望深入了解推动分子农业的复杂生物系统。此外，利用全面的蛋白质组学分析的机会已经出现，以满足良好生产规范并将源自植物的生物制药推向主流生产。
