Jiao Mengchi, Li Wenqian, Yu Yanqi, Yu Yan
Department of Chemistry, Indiana University, Bloomington, Indiana.
Biophys Rep (N Y). 2022 Mar 9;2(1). doi: 10.1016/j.bpr.2021.100041. Epub 2021 Dec 10.
Anisotropic arrangement of cell wall components is ubiquitous among bacteria and fungi, but how such functional anisotropy affects interactions between microbes and host immune cells is not known. Here we address this question with regard to phagosome maturation, the process used by host immune cells to degrade internalized microbes. We developed two-faced microparticles as model pathogens that display ligands on only one hemisphere and simultaneously function as fluorogenic sensors for probing biochemical reactions inside phagosomes during degradation. The fluorescent indicator on just one hemisphere gives the particle sensors a moon-like appearance. We show that anisotropic presentation of ligands on particles delays the start of acidification and proteolysis in phagosomes, but does not affect their degradative capacity. Our work suggests that the spatial presentation of ligands on pathogens plays a critical role in modulating the degradation process in phagosomes during host-pathogen interactions.
细胞壁成分的各向异性排列在细菌和真菌中普遍存在，但这种功能上的各向异性如何影响微生物与宿主免疫细胞之间的相互作用尚不清楚。在这里，我们针对吞噬体成熟这一宿主免疫细胞用于降解内化微生物的过程来解决这个问题。我们开发了双面微粒作为模型病原体，它们仅在一个半球上展示配体，同时作为荧光传感器，用于探测降解过程中吞噬体内的生化反应。仅一个半球上的荧光指示剂使颗粒传感器呈现出月亮般的外观。我们表明，颗粒上配体的各向异性呈现会延迟吞噬体中酸化和蛋白水解的开始，但不影响其降解能力。我们的工作表明，病原体上配体的空间呈现在宿主 - 病原体相互作用期间调节吞噬体中的降解过程中起着关键作用。
