School of Medicine and Pharmaceutical Engineering, Institute of Applied Biotechnology, Taizhou Vocational and Technical College, Taizhou, 318000, China.
Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China.
Biotechnol Lett. 2022 Apr;44(4):623-633. doi: 10.1007/s10529-022-03248-9. Epub 2022 Apr 6.
Shellfish waste is a primary source for making N-acetyl-D-glucosamine. Thus, establishing a high-efficiency and low-cost bioconversion method to produce N-acetyl-D-glucosamine directly from shellfish waste was promising.
A mutant C81 was obtained from Chitinolyticbacter meiyuanensis SYBC-H1 via Co-γ irradiation. This mutant C81 showed the highest chitinase activity of 9.8 U/mL that was 85% higher than the parent strain. The mutant C81 exhibted improved antioxidant activities, including total antioxidant capacity, superoxide radical ability, and hydroxyl radical scavenging ability, compared to that of the parent strain. Four out of nine organic solvents increased the chitinase activity by 1.9%, 6.8%, 11.7%, and 15.8%, corresponding to methylbenzene, n-heptane, petroleum ether, and n-hexane, respectively. The biphase system composed of aqueous and hexane presented a five-fold reduction of cell viability compared to the control. Using a continuous fermentation bioconversion process, 4.2 g/L GlcNAc was produced from crayfish shell powder with a yield of 80% of the chitin content.
This study demonstrated that the mutant C81 is suitable for converting crayfish shell powder into GlcNAc in an aqueous-organic system.
贝类废物是生产 N-乙酰-D-葡萄糖胺的主要原料。因此，建立一种高效、低成本的生物转化方法，直接从贝类废物生产 N-乙酰-D-葡萄糖胺是有前景的。
通过 Co-γ 辐照从几丁质分解菌 SYBC-H1 中获得突变体 C81。该突变体 C81 的几丁质酶活性最高，达到 9.8 U/mL，比亲株高 85%。与亲株相比，突变体 C81 的抗氧化活性得到了提高，包括总抗氧化能力、超氧自由基能力和羟基自由基清除能力。九种有机溶剂中有四种使几丁质酶活性分别提高了 1.9%、6.8%、11.7%和 15.8%，对应于苯、正庚烷、石油醚和正己烷。与对照相比，由水相和己烷组成的双相体系使细胞活力降低了五倍。利用连续发酵生物转化工艺，从小龙虾壳粉中生产出 4.2 g/L 的 GlcNAc，产率为壳聚糖含量的 80%。
本研究表明，突变体 C81 适合在水-有机体系中转化小龙虾壳粉为 GlcNAc。
