College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
Cell Stress Chaperones. 2022 May;27(3):285-293. doi: 10.1007/s12192-022-01270-4. Epub 2022 Apr 6.
At present, heat stress caused by the thermal environment is the main factor that endangers the reproductive function of animals. Growth hormone (GH) is a polypeptide hormone, the biological function of reproductive organs has been reported, and it has many important physiological functions in the body. However, so far, the behavior and signal transduction of GH in testicular cells under heat stress are still unclear. To this end, in the current work, we use a swine testicular cell line (ST) as an in vitro model to explore the cell behavior and intracellular signaling profile of porcine growth hormone (pGH) under heat stress; the results showed that when cells were under heat stress, pGH and GHR were basically not internalized, and a large number of them accumulated on the cell membrane. In addition, we also studied the effect of pGH on the JAK2-STATs signaling pathway and IGF-1 expression under heat stress, we found that the ability of pGH to activate the JAK-STATs signaling pathway and IGF-1 under heat stress was greatly reduced (p < 0.05). In conclusion, our research shows that when cells undergo heat stress, the internalization of pGH and GHR were inhibited, and the activation of the JAK2-STATs signaling pathway and IGF-1 expression were reduced; this lays a solid foundation for further research on the effect of pGH on swine testicular tissue under thermal environment.
目前，热环境引起的热应激是危害动物生殖功能的主要因素。生长激素（GH）是一种多肽激素，已报道其对生殖器官具有生物功能，它在体内具有许多重要的生理功能。然而，迄今为止，GH 在睾丸细胞中的热应激下的行为和信号转导仍不清楚。为此，在目前的工作中，我们使用猪睾丸细胞系（ST）作为体外模型，探讨热应激下猪生长激素（pGH）在睾丸细胞中的细胞行为和细胞内信号谱；结果表明，当细胞处于热应激时，pGH 和 GHR 基本上没有内化，大量的 pGH 和 GHR 积聚在细胞膜上。此外，我们还研究了 pGH 在热应激下对 JAK2-STATs 信号通路和 IGF-1 表达的影响，发现 pGH 在热应激下激活 JAK-STATs 信号通路和 IGF-1 的能力大大降低（p < 0.05）。总之，我们的研究表明，当细胞发生热应激时，pGH 和 GHR 的内化受到抑制，JAK2-STATs 信号通路的激活和 IGF-1 的表达减少；这为进一步研究热环境下 pGH 对猪睾丸组织的影响奠定了坚实的基础。
