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干燥的 在重新水合后,使用葡萄糖在黑暗条件下表现出固氮活性。

Dried exhibits nitrogen-fixing activity using glucose under dark conditions after rehydration.

机构信息

Science, Kyoto University, Otsu, Japan.

Agriculture, Ryukoku University, Otsu, Japan.

出版信息

Plant Signal Behav. 2022 Dec 31;17(1):2059251. doi: 10.1080/15592324.2022.2059251.

DOI:10.1080/15592324.2022.2059251
PMID:35384797
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8993094/
Abstract

is an edible cyanobacterium that produces a massive gelatinous polysaccharide matrix around the filamentous cells. The polysaccharides, more than 70% of which comprise glucose, are essential for resistance to environmental stresses. In the present study, we collected naturally growing colonies, dried them for preservation, rehydrated them, and then examined their nitrogen-fixing activity using the acetylene reduction method. As expected, the rehydrated performed nitrogen fixation after illumination with white light. Notably, under dark, aerobic conditions, the rehydrated exhibited nitrogen fixation in the presence of glucose. In contrast, under dark, anaerobic conditions, nitrogen fixation was low. Because the natural habitats of are aerobic but lack carbohydrates, cells may exhibit glucose utilization activity constitutively.

摘要

地木耳是一种可食用的蓝藻,它在丝状细胞周围产生大量的胶状多糖基质。多糖中超过 70%的成分是葡萄糖,这对于抵抗环境压力至关重要。在本研究中,我们收集了自然生长的地木耳菌落,将其干燥保存,然后在重新水化后使用乙炔还原法检测其固氮活性。不出所料,重新水化的地木耳在白光照射下进行固氮。值得注意的是,在黑暗、需氧条件下,存在葡萄糖的情况下,重新水化的地木耳也表现出固氮作用。相比之下,在黑暗、厌氧条件下,固氮作用较低。由于地木耳的自然栖息地是需氧的,但缺乏碳水化合物,因此地木耳细胞可能会持续表现出对葡萄糖的利用活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7ac/8993094/156351684aba/KPSB_A_2059251_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7ac/8993094/017f5050a739/KPSB_A_2059251_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7ac/8993094/156351684aba/KPSB_A_2059251_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7ac/8993094/017f5050a739/KPSB_A_2059251_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7ac/8993094/156351684aba/KPSB_A_2059251_F0002_OC.jpg

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