el-Demiry M I, Hargreave T B, Busuttil A, James K, Chisholm G D
Urology. 1986 Dec;28(6):492-6. doi: 10.1016/0090-4295(86)90150-0.
An indirect immunoperoxidase technique employing specific monoclonal antibodies has been used to identify leucocyte subpopulations in cytocentrifuge smears of washed human ejaculate. Cells reacting with the pan antihuman leucocyte monoclonal antibody (HLe-1) were demonstrated in 63/67 specimens from subfertile patients with a mean count of 14.5 +/- 17.1 leucocytes per HPF (X 320). Cells with similar reactivity were observed in all specimens examined from 10 fertile men with a mean count of 41.6 +/- 26.3 leucocytes per HPF (X 320). Leu-T4+ cells (T-lymphocytes) were demonstrated in only 13/63 of the subfertile group with a mean count of 4.46 +/- 3.3 T-lymphocytes per HPF (X 320). Studies with the anti-leu 2a antibody revealed that these leu-4+ cells were mainly of the suppressor/cytotoxic phenotype. In contrast, no leu-4+ cells were detected in the control group. No leu-12+ cells (B-lymphocytes) were detected in any of the 80 specimens examined.
一种采用特异性单克隆抗体的间接免疫过氧化物酶技术已被用于鉴定经洗涤的人类精液细胞离心涂片上的白细胞亚群。在67例不育患者的63份标本中发现了与泛抗人白细胞单克隆抗体（HLe-1）发生反应的细胞，每高倍视野（×320）白细胞平均计数为14.5±17.1。在10例生育男性的所有检测标本中均观察到具有类似反应性的细胞，每高倍视野（×320）白细胞平均计数为41.6±26.3。在不育组的63例中仅有13例检测到Leu-T4+细胞（T淋巴细胞），每高倍视野（×320）T淋巴细胞平均计数为4.46±3.3。用抗leu 2a抗体进行的研究表明，这些leu-4+细胞主要具有抑制/细胞毒性表型。相比之下，在对照组中未检测到leu-4+细胞。在所检测的80份标本中，均未检测到leu-12+细胞（B淋巴细胞）。
