Banliat Charles, Mahé Coline, Lavigne Régis, Com Emmanuelle, Pineau Charles, Labas Valérie, Guyonnet Benoit, Mermillod Pascal, Saint-Dizier Marie
CNRS, INRAE, Université de Tours, IFCE, UMR PRC, Nouzilly, France.
Union Evolution, Noyal-sur-Vilaine, France.
Front Cell Dev Biol. 2022 Mar 21;10:863700. doi: 10.3389/fcell.2022.863700. eCollection 2022.
Early embryo development is a dynamic process involving important molecular and structural changes leading to the embryonic genome activation (EGA) and early cell lineage differentiation. Our aim was to elucidate proteomic changes in bovine embryos developed . Eleven females were used as embryo donors and pools of embryos at the 4-6 cell, 8-12 cell, morula, compact morula and blastocyst stages were analyzed by nanoliquid chromatography coupled with label free quantitative mass spectrometry. A total of 2,757 proteins were identified, of which 1,950 were quantitatively analyzed. Principal component analysis of data showed a clear separation of embryo pools according to their developmental stage. The hierarchical clustering of differentially abundant proteins evidenced a first cluster of 626 proteins that increased in abundance during development and a second cluster of 400 proteins that decreased in abundance during development, with most significant changes at the time of EGA and blastocyst formation. The main pathways and processes overrepresented among upregulated proteins were RNA metabolism, protein translation and ribosome biogenesis, whereas Golgi vesicle transport and protein processing in endoplasmic reticulum were overrepresented among downregulated proteins. The pairwise comparison between stages allowed us to identify specific protein interaction networks and metabolic pathways at the time of EGA, morula compaction and blastocyst formation. This is the first comprehensive study of proteome dynamics in non-rodent mammalian embryos developed . These data provide a number of protein candidates that will be useful for further mechanistic and functional studies.
早期胚胎发育是一个动态过程，涉及重要的分子和结构变化，导致胚胎基因组激活（EGA）和早期细胞谱系分化。我们的目的是阐明体外发育的牛胚胎中的蛋白质组变化。使用11头雌性作为胚胎供体，对4-6细胞、8-12细胞、桑葚胚、致密桑葚胚和囊胚阶段的胚胎池进行了纳升液相色谱结合无标记定量质谱分析。共鉴定出2757种蛋白质，其中1950种进行了定量分析。数据的主成分分析显示，胚胎池根据其发育阶段有明显的分离。差异丰富蛋白质的层次聚类表明，第一组有626种蛋白质在发育过程中丰度增加，第二组有400种蛋白质在发育过程中丰度降低，在EGA和囊胚形成时变化最为显著。上调蛋白质中过度富集的主要途径和过程是RNA代谢、蛋白质翻译和核糖体生物发生，而下调蛋白质中过度富集的是高尔基体囊泡运输和内质网中的蛋白质加工。各阶段之间的成对比较使我们能够确定EGA、桑葚胚致密化和囊胚形成时的特定蛋白质相互作用网络和代谢途径。这是首次对体外发育的非啮齿类哺乳动物胚胎蛋白质组动力学进行的全面研究。这些数据提供了许多蛋白质候选物，将有助于进一步的机制和功能研究。
