Nakagawara A, Toyohara T, Nada O, Ikeda K
Z Kinderchir. 1986 Oct;41(5):275-8. doi: 10.1055/s-2008-1043358.
The intratumorous distribution of catecholaminergic tumour cells and S100 protein-positive elements of 8 neuroblastomas, 8 ganglioneuroblastomas and 2 ganglioneuromas were studied using catecholamine fluorescence and immunohistochemical staining, respectively. The elements of catecholamine fluorescence were observed in all tumour specimens, even in urinary VMA-negative cases, but the patterns of distribution were not uniform. Catecholamine fluorescence was observed in both tumour cell bodies and neurofibres, and the appearance of the latter but not that of the former correlated with the histological grade of differentiation, thereby suggesting the occurrence of catecholaminergic differentiation within the tumour. S100 protein-positive elements also correlated with the histological differentiation, and were distributed mostly in the area where the catecholamine-containing neurofibres were located. The possible correlation between catecholaminergic differentiation and the appearance of S100 protein-positive elements in neuroblastoma requires close attention.
分别采用儿茶酚胺荧光法和免疫组织化学染色法，研究了8例神经母细胞瘤、8例神经节神经母细胞瘤和2例神经节瘤中儿茶酚胺能肿瘤细胞和S100蛋白阳性成分的瘤内分布情况。在所有肿瘤标本中均观察到儿茶酚胺荧光成分，即使在尿香草扁桃酸（VMA）阴性的病例中也是如此，但分布模式并不均匀。在肿瘤细胞体和神经纤维中均观察到儿茶酚胺荧光，后者而非前者的出现与组织学分化程度相关，从而提示肿瘤内发生了儿茶酚胺能分化。S100蛋白阳性成分也与组织学分化相关，且大多分布在含儿茶酚胺神经纤维所在区域。神经母细胞瘤中儿茶酚胺能分化与S100蛋白阳性成分出现之间的可能相关性值得密切关注。
