Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine.
Laboratory of Experimental Toxicology and Mutagenesis, L.I. Medved's Research Center of Preventive Toxicology, Food and Chemical Safety, MHU, Kyiv, Ukraine.
Arch Insect Biochem Physiol. 2022 Aug;110(4):e21893. doi: 10.1002/arch.21893. Epub 2022 Apr 6.
Glyphosate-based herbicide Roundup, as the most employed herbicide used for multiple purposes in agriculture, adversely affects nontarget organisms. We tested the effects of Roundup applied at larval and adult stages. Roundup caused developmental delay and increased larvae mortality. Roundup treatment reduced hemolymph glucose and glycogen levels in adult flies of both sexes at the highest concentration tested. Sex-dependent diverse effects were found in catalase and Cu,Zn superoxide dismutase (Cu,Zn-SOD) activities. Decreased aconitase activity, contents of thiols, and lipid peroxides were found after larval Roundup exposure. Furthermore, chronic exposure to adult flies decreased appetite, body weight, and shortened lifespan. Thus, our results suggest that high concentrations of Roundup are deleterious to both larvae and adults, resulting in a shift of the metabolism and antioxidant defense system in Drosophila melanogaster.
草甘膦除草剂“农达”，作为农业中用途广泛的最常用除草剂之一，对非靶标生物具有不良影响。我们测试了在幼虫和成虫阶段施用“农达”的效果。“农达”导致幼虫发育延迟和死亡率增加。在测试的最高浓度下，“农达”处理降低了雌雄蝇成虫血淋巴中的葡萄糖和糖原水平。在两性中发现了依赖于性别的不同的过氧化氢酶和铜锌超氧化物歧化酶（Cu，Zn-SOD）活性的差异。在幼虫暴露于“农达”后，发现柠檬酸合酶活性、巯基含量和脂质过氧化物降低。此外，慢性暴露于成年果蝇会降低食欲、体重和缩短寿命。因此，我们的结果表明，高浓度的“农达”对幼虫和成虫都是有害的，导致黑腹果蝇的新陈代谢和抗氧化防御系统发生转变。
