Maldonado C A, Aoki A
Basic Appl Histochem. 1986;30(3):301-5.
Immunolabelling of prolactin (PRL) with protein A-colloidal gold complex and tissue fine structure were enhanced after postfixation of pituitary gland with osmium tetroxide and embedment in acrylic momers (LR White). Thin sections were treated with sodium metaperiodate before immunocytochemistry. An intense PRL labelling was detected in secretory granules, Golgi complexes and extracellular accumulation of the hormone. The use of osmium greatly improved the fine structure of the tissue and its stability during acrylic embedment.
用四氧化锇对垂体进行后固定并包埋于丙烯酸单体（LR White）中后，催乳素（PRL）与蛋白A-胶体金复合物的免疫标记及组织精细结构得到增强。在免疫细胞化学之前，对薄切片用偏高碘酸钠处理。在分泌颗粒、高尔基体复合物及该激素的细胞外聚积物中检测到强烈的PRL标记。锇的使用极大地改善了组织的精细结构及其在丙烯酸包埋过程中的稳定性。
