Department of Zoology, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece. 2Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece. .
Department of Zoology, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece. .
Zootaxa. 2021 Dec 22;5082(6):572-582. doi: 10.11646/zootaxa.5082.6.4.
For the genus Diaphanosoma Fischer (Cladocera) the species name D. brachyurum has been widely used for many decades to identify other species belonging to this genus. To clarify the diversity of the genus in Greek lakes in the present study, we morphologically and genetically identified the Diaphanosoma species occurring in eight lakes. Three hundred twenty-nine Diaphanosoma individuals were morphologically examined, while for the genetic analyses the mtDNA COI gene was sequenced in 48 individuals. Combining the morphological and genetic results, we verified the occurrence of D. mongolianum, D. orghidani and D. macedonicum in our study area. We could not confirm prior records of D. brachyurum and D. lacustris while we provide the molecular identity of D. macedonicum. Furthermore, we highlight the need to check whether the European D. mongolianum populations are characterised of mitochondrial discordance and hybridization as the individuals from the Asian type locality of the species. Our results support the importance of combining both approaches to correctly identify taxonomic species, despite the extra effort and cost during the sample analysis.
对于 Diaphanosoma Fischer 属（枝角目），几十年来，物种名称 D. brachyurum 被广泛用于识别该属的其他物种。为了阐明希腊湖泊中该属的多样性，本研究通过形态学和遗传学方法鉴定了 8 个湖泊中出现的 Diaphanosoma 物种。我们对 329 只 Diaphanosoma 个体进行了形态学检查，而对于遗传分析，我们对 48 只个体的 mtDNA COI 基因进行了测序。通过结合形态学和遗传学结果，我们验证了 D. mongolianum、D. orghidani 和 D. macedonicum 在我们研究区域的存在。我们无法证实先前关于 D. brachyurum 和 D. lacustris 的记录，同时我们提供了 D. macedonicum 的分子身份。此外，我们强调需要检查欧洲 D. mongolianum 种群是否具有线粒体不和谐和杂交的特征，就像来自该物种亚洲型地点的个体一样。我们的研究结果支持结合这两种方法来正确识别分类物种的重要性，尽管在样品分析过程中需要额外的努力和成本。
