Li Tingting, Cao Sumei, Liao Xiudong, Shao Yuxin, Zhang Liyang, Lu Lin, Liu Zongping, Luo Xugang
Poultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Mineral Nutrition Research Division, State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Vet Sci. 2022 Mar 22;9:855405. doi: 10.3389/fvets.2022.855405. eCollection 2022.
Understanding the underlying mechanisms that regulate the bone phosphorus (P) utilization would be helpful for developing feasible strategies to improve utilization efficiency of P in poultry. We aimed to investigate the effects of inorganic P levels on P utilization, local bone-derived regulators and bone morphogenetic protein/mitogen-activated protein kinase (BMP/MAPK) pathway in primary cultured osteoblasts of broiler chicks in order to address whether local bone-derived regulators or BMP/MAPK pathway was involved in regulating the bone P utilization of broilers using an model. The primary cultured tibial osteoblasts of broiler chicks were randomly divided into one of five treatments with six replicates for each treatment. Then, cells were respectively incubated with 0.0, 0.5, 1.0, 1.5, or 2.0 mmol/L of added P as NaHPO for 24 days. The results showed that as added P levels increased, tibial osteoblastic P retention rate, number and area of mineralized nodules, the mRNA expressions of endopeptidases on the X chromosome (), dentin matrix protein 1 (), bone morphogenetic protein 2 (), and the mRNA and protein expressions of matrix extracellular phosphoglycoprotein (MEPE) increased linearly ( < 0.001) or quadratically ( < 0.04), while extracellular signal-regulated kinase 1 () mRNA expression and c-Jun N-terminal kinase 1 (JNK1) phosphorylated level decreased linearly ( < 0.02) or quadratically ( < 0.01). Correlation analyses showed that tibial osteoblastic P retention rate was positively correlated (r = 0.452-0.564, < 0.03) with and mRNA expressions. Furthermore, both number and area of mineralized nodules were positively correlated (r = 0.414-0.612, < 0.03) with , and mRNA expressions but negatively correlated (r = -0.566 to -0.414, < 0.04) with the mRNA expression and JNK1 phosphorylated level. These results suggested that P utilization in primary cultured tibial osteoblasts of broiler chicks might be partly regulated by PHEX, DMP1, MEPE, BMP2, ERK1, and JNK1.
了解调节骨骼磷（P）利用的潜在机制，将有助于制定可行的策略来提高家禽磷的利用效率。我们旨在研究无机磷水平对肉鸡雏鸡原代培养成骨细胞中磷利用、局部骨源性调节因子和骨形态发生蛋白/丝裂原活化蛋白激酶（BMP/MAPK）信号通路的影响，以便使用模型探讨局部骨源性调节因子或BMP/MAPK信号通路是否参与调节肉鸡的骨骼磷利用。将肉鸡雏鸡的原代培养胫骨成骨细胞随机分为五种处理之一，每种处理六个重复。然后，细胞分别用0.0、0.5、1.0、1.5或2.0 mmol/L添加磷（以NaHPO形式）孵育24天。结果表明，随着添加磷水平的增加，胫骨成骨细胞的磷保留率、矿化结节的数量和面积、X染色体上的内肽酶（）、牙本质基质蛋白1（）、骨形态发生蛋白2（）的mRNA表达以及基质细胞外磷酸糖蛋白（MEPE）的mRNA和蛋白表达呈线性增加（<0.001）或二次增加（<0.04），而细胞外信号调节激酶1（）mRNA表达和c-Jun氨基末端激酶1（JNK1）磷酸化水平呈线性降低（<0.02）或二次降低（<0.01）。相关性分析表明，胫骨成骨细胞的磷保留率与和mRNA表达呈正相关（r = 0.452 - 0.564，<0.03）。此外，矿化结节的数量和面积均与、和mRNA表达呈正相关（r = 0.414 - 0.612，<0.03），但与mRNA表达和JNK1磷酸化水平呈负相关（r = -0.566至-0.414，<0.04）。这些结果表明，肉鸡雏鸡原代培养胫骨成骨细胞中的磷利用可能部分受PHEX、DMP-1、MEPE、BMP-2、ERK1和JNK1的调节。
