Pharmacy Department, Qingdao Mental Health Center, Qingdao University, No. 299 Nanjing Road, Qingdao 266000, Shandong, China.
Outpatient Department, Qingdao Mental Health Center, Qingdao University, No. 299 Nanjing Road, Qingdao 266000, Shandong, China.
Physiol Behav. 2022 Jun 1;250:113800. doi: 10.1016/j.physbeh.2022.113800. Epub 2022 Apr 5.
Herb-derived therapeutics is an attractive strategy to treat depression. Here we report the ameliorating effects of Sulfuretin, an anti-inflammatory compound in a depressive mouse model.
Immobility times were obtained in the tail suspension test and forced swim test performed from day 14 to day 16. Quantitative real-time PCR (qRT-PCR) and Western blot were used to measure brain-derived neurotrophic factor (BDNF) and the extracellular signal-regulated kinase (ERK) pathway of the hippocampus tissue on day 17. SL327 was used to block the ERK pathway in mice to evaluate the interaction between Sulfuretin and the ERK pathway. Mice were treated with Sulfuretin for 14 days before lipopolysaccharide (LPS) injection (0.83 mg/kg/day, i.p.) for two days.
Behavior tests showed that Sulfuretin dose-dependently decreased immobility times correlated with depression symptoms. BDNF levels and ERK signaling were significantly restored in the Sulfuretin-treated mice, showing the improvement of brain function. Blocking the p-ERK signaling abrogated the effects of Sulfuretin in improving behaviors and levels of BDNF.
Our study suggests that Sulfuretin exhibits anti-depressive function in LPS-induced depressive mice, in which the ERK signaling plays an essential role.
草药疗法是治疗抑郁症的一种有吸引力的策略。在这里，我们报告了一种抗炎化合物 Sulfuretin 在抑郁小鼠模型中的改善作用。
在第 14 天至第 16 天进行的悬尾试验和强迫游泳试验中，记录不动时间。第 17 天，使用定量实时 PCR（qRT-PCR）和 Western blot 测量海马组织中的脑源性神经营养因子（BDNF）和细胞外信号调节激酶（ERK）通路。使用 SL327 阻断小鼠的 ERK 通路，以评估 Sulfuretin 与 ERK 通路之间的相互作用。在 LPS（0.83mg/kg/天，腹腔注射）注射前两天，用 Sulfuretin 处理小鼠 14 天。
行为测试表明，Sulfuretin 呈剂量依赖性地减少与抑郁症状相关的不动时间。Sulfuretin 处理的小鼠中 BDNF 水平和 ERK 信号显著恢复，表明大脑功能得到改善。阻断 p-ERK 信号会消除 Sulfuretin 改善行为和 BDNF 水平的作用。
我们的研究表明，Sulfuretin 对 LPS 诱导的抑郁小鼠具有抗抑郁作用，其中 ERK 信号起重要作用。
