Kim Gyeongjin, Gil Dongkyun, Lee Jae Jun, Kim Jiwon, Kim Ki-Tae, Kim Cheal
Department of Fine Chem. and New and Renewable Energy Convergence, Seoul National Univ. of Sci. and Tech. (SNUT), Seoul 01811, Republic of Korea.
Department of Environ. Engineering, Seoul National Univ. of Sci. and Tech. (SNUT), Seoul 01811, Republic of Korea.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Aug 5;276:121207. doi: 10.1016/j.saa.2022.121207. Epub 2022 Mar 31.
A novel 7-nitro-1,2,3-benzoxadiazole (NBD)-based chemosensor BOP ((5-bromopyridin-2-yl)(4-(7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)piperazin-1-yl)methanone) was synthesized. BOP could detect S through fluorescent quenching and colorimetric change. The detection limit was calculated to be 10.9 µM through fluorescence titration. The reaction mechanism of BOP towards S was estimated to be thiolysis of NBD amine, producing the cleavage products, NBD-S and BP ((5-bromopyridin-2-yl)(piperazin-1-yl)methanone). The thiolysis was demonstrated by H NMR titrations, ESI-mass analysis and theoretical calculations. Importantly, BOP was able to successfully monitor S in zebrafish and water samples. Additionally, test strips coated with BOP were applied to the in-the-field measurements of S.
合成了一种新型的基于7-硝基-1,2,3-苯并恶二唑（NBD）的化学传感器BOP（(5-溴吡啶-2-基)(4-(7-硝基苯并[c][1,2,5]恶二唑-4-基)哌嗪-1-基)甲酮）。BOP可通过荧光猝灭和比色变化检测硫。通过荧光滴定计算出检测限为10.9 μM。估计BOP与硫的反应机理为NBD胺的硫解，生成裂解产物NBD-S和BP（(5-溴吡啶-2-基)(哌嗪-1-基)甲酮）。通过1H NMR滴定、电喷雾电离质谱分析和理论计算证实了硫解反应。重要的是，BOP能够成功监测斑马鱼和水样中的硫。此外，涂有BOP的测试条被用于硫的现场测量。
