[滤泡辅助性T细胞和B细胞在人类慢性移植物抗宿主病中的作用及机制]
[The Role and Mechanism of Tfh and B Cell in Human Chronic Graft-Versus-Host-Disease].
作者信息
Lyu Kang-Kang, Xu Mi-Mi, DU Yuan-Yuan, He Xue-Feng, Chen Feng, Ma Xiao, Han Yue, Wu De-Pei, Liu Yue-Jun, Xu Ming-Zhu
机构信息
National Clinical Research Center for Hematologic Diseases, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Institute of Blood and Marrow Transplantation, Collaborative Innovation Center of Hematology, Soochow University, Suzhou 215006, Jiangsu Province, China.
National Clinical Research Center for Hematologic Diseases, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Institute of Blood and Marrow Transplantation, Collaborative Innovation Center of Hematology, Soochow University, Suzhou 215006, Jiangsu Province, China,E-mail:
出版信息
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2022 Apr;30(2):593-599. doi: 10.19746/j.cnki.issn.1009-2137.2022.02.045.
UNLABELLED
AbstractObjective: To investigate the role and mechanism of circulating follicular helper T cells (cTfh), extrafollicular helper T cells, B cells and their subsets in chronic graft-versus-host disease (cGVHD) after transplantation.
METHODS
Peripheral blood of cGVHD 64 patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT) at the First Affiliated Hospital of Soochow University from 2016 to 2019 were collected. The percentage of cTfh cells, extrafollicular helper T cells, B cells and subsets were detected by flow cytometry. The healthy donors were detected as control. Percentage of each cell subpopulation between the two groups were compared by the two-tailed Students' T test.
RESULTS
The percentage of circulating follicular helper T cells (cTfh, CD4CXCR5) was markedly decreased in patients with cGVHD as compared with that in the healthy donors (0.53%±0.10% vs 3.91%±0.60%, P<0.001). The percentage of extrafollicular helper T cells (CD44CD62LPSGL-1CD4T) of the patients in cGVHD and the healthy donors were 8.86%±0.45% and 5.38%±0.79% (P=0.003). A significant change in B cell subsets was found in the patients with cGVHD. The two types of antigen-stimulate CD27 B cell: the percentages of pre-GC B cells (CD19IgDCD38CD27) and plasmablast/plasma cells (PB/PC, CD19IgDCD38CD27) of patients with cGVHD were 20.91%±2.70% and 41.05%±5.00%, respectively, which were significantly higher than those in healthy donors (P=0.005, P=0.014). Meanwhile, the percentage of unstimulated CD27 B cells in patients with cGVHD was significantly reduced, especially the naive B cells (CD19IgDCD38CD27, 12.59%±2.63%, P=0.025). There was a positive correlation between the percentage of extrafollicular helper T cells and plasmablast/ plasma cells (PB/PC) in cGVHD patients (r=0.43).
CONCLUSION
Compared with healthy donor, cTfh cells, extrafollicular helper T cells and B cell subsets in the peripheral blood of patients with cGVHD after transplantation changed in varying degress.
未标记
摘要目的：探讨循环滤泡辅助性T细胞（cTfh）、滤泡外辅助性T细胞、B细胞及其亚群在移植后慢性移植物抗宿主病（cGVHD）中的作用及机制。
方法
收集2016年至2019年苏州大学附属第一医院64例异基因造血干细胞移植（allo-HSCT）后cGVHD患者的外周血。采用流式细胞术检测cTfh细胞、滤泡外辅助性T细胞、B细胞及其亚群的百分比。检测健康供者作为对照。两组间各细胞亚群的百分比采用双侧Student's T检验进行比较。
结果
与健康供者相比，cGVHD患者循环滤泡辅助性T细胞（cTfh，CD4CXCR5）的百分比显著降低（0.53%±0.10%对3.91%±0.60%，P<0.001）。cGVHD患者和健康供者的滤泡外辅助性T细胞（CD44CD62LPSGL-1CD4T）百分比分别为8.86%±0.45%和5.38%±0.79%（P=0.003）。发现cGVHD患者的B细胞亚群有显著变化。两种抗原刺激的CD27 B细胞：cGVHD患者的前生发中心B细胞（CD19IgDCD38CD27）和浆母细胞/浆细胞（PB/PC，CD19IgDCD38CD27）百分比分别为20.91%±2.70%和41.05%±5.00%，显著高于健康供者（P=0.005，P=0.014）。同时，cGVHD患者未刺激的CD27 B细胞百分比显著降低，尤其是幼稚B细胞（CD19IgDCD38CD27，12.59%±2.63%，P=0.025）。cGVHD患者中滤泡外辅助性T细胞百分比与浆母细胞/浆细胞（PB/PC）之间存在正相关（r=0.43）。
结论
与健康供者相比，移植后cGVHD患者外周血中的cTfh细胞、滤泡外辅助性T细胞和B细胞亚群有不同程度的变化。
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