Bao Binghao, Yang Zhen, Deng Sheng, Dai Hengheng, Feng Junlong, Meng Fanchao, Li Haisong, Wang Jisheng
Graduate School of Beijing University of Chinese Medicine, Beijing, China.
Andrology Department, China-Japan Friendship Hospital, Beijing, China.
Andrologia. 2022 Jul;54(6):e14407. doi: 10.1111/and.14407. Epub 2022 Apr 8.
Zuogui Wan (ZGW) is a common prescription medication used in traditional Chinese medicine (TCM) to significantly improve the sperm quality and treat male infertility. This study evaluated the repair effect of ZGW and Levocarnitine (LEV) on GC1-spg cell injury induced by Glucosides of Tripterygium WilforDII Hook (GTW). The results showed that the ultrastructure and apoptosis rate of GC1- spg cells in LEV and ZGW group were considerably better than GTW. The transcriptional and translational level of CYP1A1, CYP17A1, androgen receptor (AR), SRD5A2 and proliferating cell nuclear antigen (PCNA) in GC-1spg cells of the LEV group were considerably elevated than GTW group (p < 0.05 or 0.01). Furthermore, the transcriptional and translational levels of CYP19A1, CYP17A1, AR, SRD5A2 and PCNA in GC-1spg cells in ZGW group were found to be considerably elevated than the LEV group (p < 0.05 or 0.01). The findings indicate that ZGW and LEV could increase the expression of PCNA, CYP17A1, CYP19A1, SRD5A2 and AR at transcriptional and translational levels, inhibit GC-1spg cell apoptosis and promoting cell proliferation, and the effect of ZGW was found to be significantly better than that of LEV.
左归丸（ZGW）是一种常用的中药处方药，可显著改善精子质量并治疗男性不育症。本研究评估了左归丸和左卡尼汀（LEV）对雷公藤多苷（GTW）诱导的GC1-spg细胞损伤的修复作用。结果表明，左卡尼汀和左归丸组GC1-spg细胞的超微结构和凋亡率明显优于雷公藤多苷组。左卡尼汀组GC-1spg细胞中CYP1A1、CYP17A1、雄激素受体（AR）、SRD5A2和增殖细胞核抗原（PCNA）的转录和翻译水平明显高于雷公藤多苷组（p < 0.05或0.01）。此外，发现左归丸组GC-1spg细胞中CYP19A1、CYP17A1、AR、SRD5A2和PCNA的转录和翻译水平明显高于左卡尼汀组（p < 0.05或0.01）。研究结果表明，左归丸和左卡尼汀可在转录和翻译水平上增加PCNA、CYP17A1、CYP19A1、SRD5A2和AR的表达，抑制GC-1spg细胞凋亡并促进细胞增殖，且左归丸的效果明显优于左卡尼汀。
