Department of Hygiene Inspection & Quarantine Science, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, P. R. China.
J Sep Sci. 2022 Jul;45(13):2366-2374. doi: 10.1002/jssc.202200106. Epub 2022 Apr 14.
This study is aimed to establish a simple, rapid, and accurate ion chromatography approach for the simultaneous detection of six inorganic anions in urine. Various performance parameters affecting the determination of anions were optimized, including the selection of sample protein precipitation agent, eluent, and flow rate. The final eluent was 3.6 mmol/L sodium carbonate and 12% isopropanol with a flow rate of 0.6 mL/min. Acetonitrile was used for pretreatment to precipitate proteins, and the volume ratio of urine to acetonitrile was 1:4. The correlation coefficient of the target anion calibration curve ranged from 0.9973 to 0.9999. The limit of detection ranged from 1.50 to 12.0 μg/L, and the method detection limit ranged from 15.0 to 120 μg/L. The standard recovery rate for low, medium, and high concentrations ranged from 90 to 110%. The inter-day and intra-day relative standard deviations were <5%. The method has high accuracy and good reproducibility and is suitable for the separation and determination of anions in urine.
本研究旨在建立一种简单、快速、准确的离子色谱法，用于同时检测尿液中的六种无机阴离子。优化了影响阴离子测定的各种性能参数，包括样品蛋白沉淀剂、洗脱液和流速的选择。最终的洗脱液为 3.6mmol/L 碳酸钠和 12%异丙醇，流速为 0.6mL/min。乙腈用于预处理沉淀蛋白质，尿液与乙腈的体积比为 1:4。目标阴离子校准曲线的相关系数范围为 0.9973 至 0.9999。检测限范围为 1.50 至 12.0μg/L，方法检测限范围为 15.0 至 120μg/L。低、中、高浓度的标准回收率范围为 90%至 110%。日间和日内相对标准偏差均<5%。该方法具有较高的准确性和良好的重现性，适用于尿液中阴离子的分离和测定。
