Niu Huawei, Duan Yuhan, Zhang Yuanyuan, Hua Xinting, Xu Chuan, Li Zhaozhou, Ma Jinliang, Qin Fangyuan, Zhai Yaping, Ye Yong, Zhao Yufen
National Demonstration Center for Experimental Food Processing and Safety Education, College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, 471000, China.
National Demonstration Center for Experimental Food Processing and Safety Education, College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, 471000, China.
J Photochem Photobiol B. 2022 May;230:112441. doi: 10.1016/j.jphotobiol.2022.112441. Epub 2022 Apr 4.
Most reported probes that respond to Cysteine (Cys) and Hydrogen sulfide (HS) can only identify one analyte, or they were interfered with homocysteine (Hcy) and glutathione (GSH) when recognizing Cys and HS. In addition, nitrobenzoxadiazole (NBD) ether, as one of thiols recognition sites, inevitably encounters the situation that Cys, GSH and HS cannot be distinguished on the same channel at the cellular level. In this work, by introducing NBD ether and NBD amine, we constructed a bifunctional fluorescent probe NJB for dual-site response to Cys and HS via PET & ICT processes. NJB has wonderful selectivity for identifying Cys and HS, with limits of detection as low as 58.4 nM and 81.1 nM, respectively. Interestingly, NJB has been successfully applied to detect Cys and HS in MCF-7 cells. Therefore, the probe that serves as a great tool for inquiring the physiological and pathological functions of Cys and HS in living cells is promising.
大多数已报道的对半胱氨酸（Cys）和硫化氢（HS）有响应的探针只能识别一种分析物，或者在识别Cys和HS时会受到同型半胱氨酸（Hcy）和谷胱甘肽（GSH）的干扰。此外，作为硫醇识别位点之一的硝基苯并恶二唑（NBD）醚，在细胞水平上不可避免地会遇到在同一通道上无法区分Cys、GSH和HS的情况。在这项工作中，通过引入NBD醚和NBD胺，我们构建了一种双功能荧光探针NJB，通过PET和ICT过程对Cys和HS进行双位点响应。NJB对识别Cys和HS具有出色的选择性，检测限分别低至58.4 nM和81.1 nM。有趣的是，NJB已成功应用于检测MCF-7细胞中的Cys和HS。因此，该探针作为探究活细胞中Cys和HS生理和病理功能的强大工具具有广阔前景。
