Kim Yeeun, Lee Kyung Min, Jang Guehwan, Lee Changhee
College of Veterinary Medicine and Virus Vaccine Research Center, Gyeongsang National University, Jinju, 52828, Republic of Korea.
School of Life Sciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
Arch Virol. 2022 May;167(5):1381-1385. doi: 10.1007/s00705-022-05414-w. Epub 2022 Apr 10.
Porcine hemagglutinating encephalomyelitis virus (PHEV) is a member of the subgenus Embecovirus of the genus Betacoronavirus, and it is ubiquitously distributed in most pig-farming countries worldwide with low clinical incidence. Here, we report the full-length genome sequence and molecular characterization of a novel PHEV strain identified in diarrheic neonates in South Korea. The complete genome of the Korean PHEV strain GNU-2113 was sequenced and analyzed to characterize PHEV circulating in South Korea. The GNU-2113 genome was determined to be 29,982 nucleotides in length, with large unique deletions in the regions encoding nonstructural protein 3 and NS2. It was found to share 95.1-96.9% sequence identity with other global strains. Genetic and phylogenetic analysis indicated that the GNU-2113 strain is distantly related to the existing PHEV genotypes, implying that the virus appears to undergo substantial evolution under endemic pressure. This study provides important information about the genetic diversity of PHEV circulating subclinically in swine herds, which may ensure viral fitness in the enzootic environment.
猪血凝性脑脊髓炎病毒（PHEV）是β冠状病毒属恩贝病毒亚属的成员，在全球大多数养猪国家广泛分布，但临床发病率较低。在此，我们报告在韩国腹泻新生儿中鉴定出的一种新型PHEV毒株的全基因组序列和分子特征。对韩国PHEV毒株GNU - 2113的完整基因组进行测序和分析，以表征在韩国流行的PHEV。GNU - 2113基因组长度为29,982个核苷酸，在编码非结构蛋白3和NS2的区域存在大量独特缺失。发现它与其他全球毒株的序列同一性为95.1 - 96.9%。遗传和系统发育分析表明，GNU - 2113毒株与现有的PHEV基因型关系较远，这意味着该病毒在地方流行压力下似乎经历了显著进化。本研究提供了关于在猪群中亚临床流行的PHEV遗传多样性的重要信息，这可能确保病毒在地方流行环境中的适应性。
