Feng Qi, Si Yu, Zhu Lingling, Wang Feng, Fang Junqiang, Pan Chun, Gao Xiangdong, Liu Wei
Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, PR China.
Simcere Pharmaceutical Group Limited, Nanjing, 210042, PR China.
J Ethnopharmacol. 2022 Jul 15;293:115262. doi: 10.1016/j.jep.2022.115262. Epub 2022 Apr 6.
Sarcandra glabra (Thunb.) Nakai, a valuable dietetic Chinese herb, is still widely used today. Multiple ingredients of S. glabra with a variety of activities such as anti-inflammatory, antiviral, and antitumor were studied. However, the Sarcandra glabra (Thunb.) Nakai polysaccharide hasn't been reported for its anti-inflammatory effect.
In this study, the anti-inflammatory activity of Sarcandra glabra (Thunb.) Nakai polysaccharide was assessed in LPS-induced ARDS mice.
A polysaccharide coded as SERP 30 was obtained by water extraction, alcohol precipitation, and gel filtration. After the physicochemical properties determination and structural characterization, LPS induced-mice ARDS model was used to evaluate the anti-inflammatory and associated antioxidant activities of SERP 30. H&E staining was used to observe the seriousness of lung injury in mice. The ELISA method was used to measure the expression of inflammatory factors (TNF-α and IL-6) in the serum of the mice. The TBA method and the WST-1 method were used to evaluate the oxidative stress injury. Immunohistochemistry was used to distinguish the expression of metalloproteinase-9 (MMP-9), heparinase (HPA), syndecan-1, and decorin in ARDS-mice lung tissue. Western blotting was used to confirm the expression of related proteins in mouse lung tissue.
SERP 30 had a potential role in improving lung damage, reducing inflammation, and preventing oxidative stress. Moreover, SERP 30 significantly attenuated the damage to the endothelial glycocalyx and maintained the integrity of the glycocalyx. The western blotting result implied that the main anti-inflammatory mechanism is directed towards NF-κB and MAPK signaling pathways with inhibiting the activation of associated proteins.
This research provides a theoretical basis for treating ARDS by using a byproduct from food resource.
肿节风是一种珍贵的食药两用中药材，至今仍被广泛应用。人们对肿节风的多种具有抗炎、抗病毒和抗肿瘤等多种活性的成分进行了研究。然而，肿节风多糖的抗炎作用尚未见报道。
本研究旨在评估肿节风多糖对脂多糖诱导的急性呼吸窘迫综合征（ARDS）小鼠的抗炎活性。
通过水提取、乙醇沉淀和凝胶过滤获得一种编码为SERP 30的多糖。在对其理化性质进行测定和结构表征后，采用脂多糖诱导的小鼠急性呼吸窘迫综合征模型来评估SERP 30的抗炎及相关抗氧化活性。采用苏木精-伊红（H&E）染色观察小鼠肺损伤的严重程度。采用酶联免疫吸附测定（ELISA）法检测小鼠血清中炎症因子（肿瘤坏死因子-α和白细胞介素-6）的表达。采用硫代巴比妥酸（TBA）法和水溶性四氮唑盐-1（WST-1）法评估氧化应激损伤。采用免疫组织化学法鉴别急性呼吸窘迫综合征小鼠肺组织中金属蛋白酶-9（MMP-9）、肝素酶（HPA）、多配体蛋白聚糖-1和核心蛋白聚糖的表达。采用蛋白质免疫印迹法确认小鼠肺组织中相关蛋白的表达。
SERP 30在改善肺损伤、减轻炎症和预防氧化应激方面具有潜在作用。此外，SERP 30显著减轻了对内皮糖萼的损伤并维持了糖萼的完整性。蛋白质免疫印迹结果表明，其主要抗炎机制是针对核因子-κB（NF-κB）和丝裂原活化蛋白激酶（MAPK）信号通路，抑制相关蛋白的激活。
本研究为利用食品资源副产品治疗急性呼吸窘迫综合征提供了理论依据。
