Yoon Jihee, Bae Jiyun, Kang Seulgi, Cho Byung-Kwan, Oh Min-Kyu
Department of Chemical and Biological Engineering, Korea University, Seongbuk-gu, Seoul 02841, Republic of Korea.
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea.
Bioresour Technol. 2022 Jun;353:127127. doi: 10.1016/j.biortech.2022.127127. Epub 2022 Apr 7.
Acetate is regarded as a sustainable microbial feedstock that is synthesized from biowastes such as synthesis gas (syngas), carbon dioxide, lignocellulose, or organic waste. In this study, Methylorubrum extorquens AM1 was engineered to improve the production of bioplastic poly-3-hydroxybutyrate (PHB) using acetate as the sole carbon source. To utilize acetate as a carbon source and methanol as an energy source, acs encoding acetyl-CoA synthetase and fdh from Burkholderia stabilis were overexpressed, while ftfL involved in the assimilation of methanol into formyl-tetrahydrofolate was deleted. The yields of biomass and PHB from acetate significantly improved, and the growth rate and PHB content of the bacteria increased. In addition, sustainability of the PHB production was demonstrated using acetate derived from carbon dioxide and syngas. This study shows that biopolymers could be synthesized efficiently using acetate as the sole carbon source through metabolic engineering and the supply of energy cofactors.
乙酸盐被视为一种可持续的微生物原料，它可由合成气（合成气）、二氧化碳、木质纤维素或有机废物等生物废料合成。在本研究中，对嗜甲基红球菌AM1进行了工程改造，以利用乙酸盐作为唯一碳源提高生物塑料聚-3-羟基丁酸酯（PHB）的产量。为了利用乙酸盐作为碳源和甲醇作为能源，编码乙酰辅酶A合成酶的acs和来自稳定伯克霍尔德菌的fdh被过表达，而参与将甲醇同化为甲酰四氢叶酸的ftfL被删除。乙酸盐产生的生物量和PHB产量显著提高，细菌的生长速率和PHB含量增加。此外，使用源自二氧化碳和合成气的乙酸盐证明了PHB生产的可持续性。这项研究表明，通过代谢工程和能量辅助因子的供应，可以使用乙酸盐作为唯一碳源高效合成生物聚合物。
