State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, 315211, China.
School of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Virology. 2022 May;570:117-122. doi: 10.1016/j.virol.2022.03.011. Epub 2022 Apr 4.
The small brown planthopper, Laodelphax striatellus (Hemiptera: Delphacidae), is an efficient vector of several economically important plant viruses. In this study, a novel reovirus-like virus was identified in L. striatellus, named Laodelphax striatellus reovirus (LSRV). The complete genome of LSRV was 28,207 nt, comprising of ten segments encoded 11 deduced proteins. All genome segments were conserved with AGUAA at the 5'-terminal and GUUGUC at 3'-terminal and segment-specific inverted terminal repeats. In addition, genomic characteristics and phylogenetic analysis suggested that LSRV was a new member of genus Fijivirus. Importantly, LSRV was widely distributed in various tissues and highest expressed in adult heads. However, LSRV was unable to horizontal replication in rice plants. Moreover, typical profiles of LSRV-derived small interfering RNAs indicated host antiviral RNA interference pathway was involved in LSRV infection. In conclusion, LSRV may be considered as a new species of the genus Fijivirus in the order Reovirales.
直纹稻虱，Laodelphax striatellus（半翅目：飞虱科），是几种经济上重要植物病毒的高效载体。在这项研究中，在直纹稻虱中鉴定出一种新型呼肠孤病毒样病毒，命名为直纹稻虱呼肠孤病毒（LSRV）。LSRV 的完整基因组为 28207nt，由十个编码 11 个推定蛋白的片段组成。所有基因组片段都在 5'-末端保守为 AGUAA，在 3'-末端保守为 GUUGUC，并具有特定的反向末端重复序列。此外，基因组特征和系统发育分析表明，LSRV 是 Fijivirus 属的一个新成员。重要的是，LSRV 在各种组织中广泛分布，在成虫头部的表达量最高。然而，LSRV 不能在水稻植株中进行水平复制。此外，LSRV 衍生的小干扰 RNA 的典型图谱表明，宿主抗病毒 RNA 干扰途径参与了 LSRV 的感染。总之，LSRV 可能被认为是呼肠孤病毒目 Reovirales 属的一个新种。
