Santi P A
Hear Res. 1986;24(3):179-87. doi: 10.1016/0378-5955(86)90017-1.
This paper describes a relatively rapid method (67 h) for producing surface preparations of the organ of Corti that are suitable for a computer-assisted morphometric analysis of cochlear hair cells. On day 1, a cochlea was fixed in OsO4, dehydrated in ethanol and infiltrated with an Epon-like plastic (Medcast). On day 2, the Medcast within the cochlea was polymerized at 60 degrees C in an oven. On day 3, the complete organ of Corti was dissected into approximately 20 segments which were trimmed and mounted on a slide in Medcast. Two 400 mesh transmission electron microscope grids are mounted at the apical and basal ends of the organ of Corti. These grids served as reference points for the establishment of an independent X, Y coordinate system on the slide by a computer-assisted light microscope. The segments were permanently attached to the slide by polymerization of the Medcast at 60 degrees C for approximately 15 h. At the beginning of day 4, the organ of Corti surface preparation was ready for examination by light microscopy or could be further sectioned and examined by transmission electron microscopy.
本文介绍了一种相对快速的方法（67小时）来制备柯蒂氏器的表面制剂，该制剂适用于对耳蜗毛细胞进行计算机辅助形态测量分析。第一天，将耳蜗固定在四氧化锇中，用乙醇脱水，并用一种类似环氧树脂的塑料（Medcast）浸润。第二天，耳蜗内的Medcast在60摄氏度的烤箱中聚合。第三天，将完整的柯蒂氏器解剖成大约20个节段，对这些节段进行修整并安装在载玻片上的Medcast中。在柯蒂氏器的顶端和底端各安装两个400目透射电子显微镜网格。这些网格作为通过计算机辅助光学显微镜在载玻片上建立独立的X、Y坐标系的参考点。通过在60摄氏度下将Medcast聚合大约15小时，将这些节段永久固定在载玻片上。在第四天开始时，柯蒂氏器表面制剂就可以通过光学显微镜进行检查，或者可以进一步切片并通过透射电子显微镜进行检查。
